Development and validation of class I SSR markers targeting (GATA)
n
repeat motifs in rice |
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| Authors: | Passoupathy Rajendrakumar Akshaya Kumar Biswal Kannabiran Sakthivel Maganti Sheshu Madhav Chirravuri Neeraja Sena M Balachandran Kommoju Srinivasarao Podishetty Natarajkumar Yadla Hari Kalidindi Sujatha Raman M Sundaram |
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| Institution: | (1) Biotechnology Laboratory, Crop Improvement Section, Directorate of Rice Research, Rajendranagar, Hyderabad, 500030, India |
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| Abstract: | SSR markers targeting (GATA)
n
motifs are known to be highly polymorphic and useful in many organisms. (GATA)
n
motif specific SSR markers covering the whole rice genome are not available. The present study was carried out with an objective
to identify class I rice microsatellites in the rice genome with (GATA)n motifs, in-silico, and validate their potential as molecular markers. A total of 243 such motifs were identified; 65 of these
were present in the genic region, 59 were in the upstream region and the remaining motifs were found in the intergenic regions.
Many of the (GATA)
n
motifs were found within and/or upstream of genes associated with biotic or abiotic stress tolerance. A total of 230 PCR-based
markers targeting all the class I (GATA)
n
microsatellites were developed and 35 of these markers spread across the rice genome were validated in a set of 24 representative
rice varieties belonging to five distinct cultivar groups. All the markers were polymorphic, with average polymorphism information
content (PIC) value of 0.61, and the rice cultivars could be uniquely distinguished into different cultivar groups based on
marker analysis. These informative markers targeting (GATA)
n
motifs representing a new set of markers in rice will be highly useful for genetic studies and marker-assisted selection.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
Passoupathy Rajendrakumar, Akshaya Kumar Biswal and Kannabiran Sakthivel contributed equally. |
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| Keywords: | Rice (GATA) n microsatellites Molecular markers Genetic diversity Marker-assisted selection |
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