| 抗水稻纹枯病qSB-9Tq基因效应及作用方式分析 |
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| 引用本文： | 殷跃军;左示敏**;王辉;张亚芳;陈宗祥;马玉银;顾世梁;潘学彪.抗水稻纹枯病qSB-9Tq基因效应及作用方式分析[J].作物学报,2009,35(2):279-285. |
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| 作者姓名： | 殷跃军;左示敏**;王辉;张亚芳;陈宗祥;马玉银;顾世梁;潘学彪 |
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| 作者单位： | 扬州大学江苏省作物遗传生理重点实验室/植物功能基因组学教育部重点实验室，江苏扬州225009 |
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| 基金项目： | 本研究由国家技术研究发展计划（863计划）项目（2006AA10Z165，2006AA10A103），公益性行业（农业）科研专项经费（nyhyzx07-049)资助 |
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| 摘 要： | 水稻第9染色体上存在1个抗纹枯病QTL，被命名为qSB-9，水稻品种特青在该QTL上携带抗性等位基因qSB-9Tq，而Lemont携带相对感病等位基因qSB-9Le。为精确地评价qSB-9Tq的抗病效应，分析其作用方式，利用分子标记进行前景选择和背景选择，从轮回亲本Lemont与特青回交后代群体中筛选到1个目标单株。连续3年对该单株的扩繁后代(BC6F2)及随后获得的近等基因系采用嵌入法进行接种鉴定试验。田间试验采取2种不同的设计。第一种是完全随机试验，即从BC6F2分离群体中筛选出目标区间为qSB-9TqTq纯合型、qSB-9LeLe纯合型和qSB-9TqLe杂合型个体，并对3种基因型个体间的病级平均数差异进行统计分析。第二种设计为随机区组设计，即在BC6F3和BC6F4代，分别对上述3种基因型的近等基因系群体，按3次重复的随机区组设计进行移栽和接种鉴定试验。结果表明，3年的试验结果表现出一致的趋势，即qSB-9Tq存在于分子标记RM242~Y92.5之间，可减轻病级1.0级(0~9级病情分级系统)左右，且其抗性表现为几乎完全的显性特征。本研究的结果为qSB-9Tq的精细定位和育种利用奠定了基础。
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| 关 键 词： | 水稻(Oryza sativa L.) 纹枯病 QTL 近等基因系 标记辅助选择 抗性效应 作用方式 |
| 收稿时间： | 2008-07-18 |
| 修稿时间： | 2008-10-10 |
Effect and Action Analysis of qSB-9Tq Conferring Resistance to Rice Sheath Blight |
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| YIN Yue-Jun,ZUO Shi-Min,WANG Hui,ZHANG Ya-Fang,CHEN Zong-Xiang,MA Yu-Yin,GU Shi-Liang,PAN Xue-Biao.Effect and Action Analysis of qSB-9Tq Conferring Resistance to Rice Sheath Blight[J].Acta Agronomica Sinica,2009,35(2):279-285. |
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| Authors: | YIN Yue-Jun ZUO Shi-Min WANG Hui ZHANG Ya-Fang CHEN Zong-Xiang MA Yu-Yin GU Shi-Liang PAN Xue-Biao |
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| Institution: | Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genetics,Ministry of Education,Yangzhou University, Yangzhou 22509,china |
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| Abstract: | A quantitative trait locus (QTL) contributing partial resistance to rice sheath blight (SB), named as qSB-9, has been confirmed on chromosome 9 of rice. A rice cultivar, Teqing, possesses the relatively resistant allele, qSB-9Tq, and Lemont has the relatively susceptible allele, qSB-9Le. To evaluate the resistance effect and action mode of qSB-9Tq accurately, a BC6F1 plant, from a backcross of Lemont (recurrent parent)/Teqing, with heterozygous genotype of qSB-9TqLe and similar genetic background to Lemont was screened out by using marker-assisted selection. Artificial inoculation with strong pathogenic Rhizoctonia solani line, RH-9, was conducted on the BC6F2 population and the near-isogenic lines (NILs) selected from BC6F3 and BC6F4 generation in a three-year test. Two experimental designs were adopted. One was completely randomized experiment, in which the plants with three genotypes (qSB-9TqTq, qSB-9LeLe, and qSB-9TqLe) on the locus of qSB-9 were distinguished by detecting marker genotypes flanking the qSB-9 in BC6F2 segregation population. The other was a randomized block design with 3 replicates for three NILs in BC6F3 and BC6F4 generations. Results from the two experiments all suggested that the qSB-9Tq was a dominant resistance allele in the interval between the markers of RM242 and Y92.5, and could reduce disease rating about 1.0 score under ‘0–9’ SB-disease rating system. The result of the research laid a strong foundation for the fine mapping and breeding utilization of the qSB-9Tq. |
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| Keywords: | Rice(Oryza sativa L ) Sheath blight QTL Near-isogenic line Marker-assisted selection Resistant effect Gene action |
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