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玉米小孢子培养高频再生技术程序的研究
引用本文:陆瑞菊,王亦菲,孙月芳,周润梅,沈雪芳,王义发,黄剑华.玉米小孢子培养高频再生技术程序的研究[J].中国农学通报,2005,21(2):38-38.
作者姓名:陆瑞菊  王亦菲  孙月芳  周润梅  沈雪芳  王义发  黄剑华
作者单位:(1上海市农业科学院生物技术研究中心,上海 201106;2上海市农业遗传育种重点实验室,上海 2011063上海市农业科学院作物育种栽培研究所,上海 201106)
摘    要:以大田种植的玉米供体植株为实验材料,以提高游离小孢子存活率及成胚率为重点进行了技术优化研究。摸索出了一些行之有效的技术措施,提高了游离小孢子的离体培养反应,获得了一批再生植株。通过技术优化,建立玉米小孢子离体培养高频再生技术程序,在此基础上构建了玉米DH育种技术平台。应用这一技术平台,可以在当年获得玉米DH纯系的种子,第二年即可安排大田纯系鉴定。

关 键 词:玉米  小孢子  存活率  成胚率  DH株系

Study on the Procedure of Maize Microspore Culture with Higher Frequency of Plant Regeneration
Lu Ruiju,Wang Yifei,Sun Yuefang,Zhou Runmei,Shen Xuefang,Wang Yif,Huang Jianhu.Study on the Procedure of Maize Microspore Culture with Higher Frequency of Plant Regeneration[J].Chinese Agricultural Science Bulletin,2005,21(2):38-38.
Authors:Lu Ruiju  Wang Yifei  Sun Yuefang  Zhou Runmei  Shen Xuefang  Wang Yif  Huang Jianhu
Institution:(1Agro-Biotech Research Center, Shanghai Academy of Agricultural Sciences, Shanghai 201106; 2Shanghai Key Lab. of Agricultural Genetics and Breeding; Shanghai 201106 3Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Scie
Abstract:Donor maize plants grown in the fields was used as experimental materials, study of technique optimization was made mainly for improving freq. of microspore survival and ELS formation. The efficient technique for the microspore culture was developed and the response of in vitro culture of isolated microspores was improved. The plantlets were regenerated from the culture. The procedure of isolated microspore culture with higher regeneration was established by the technique optimization and the platform of maize DH breeding was developed. The DH lines of maize was able to be obtained within 1 year and the field test for DH lines could be arranged in the nest year.
Keywords:Zea mays L  zz  Microsporezz  Freq  of survival microsporezz  Freq  of ELSzz  DH linezz
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