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| 小麦全蚀病菌病毒的研究 | |
| 引用本文： | 梁平彦,陈开英.小麦全蚀病菌病毒的研究[J].植物病理学报,1984,14(3):159-164. |
| 作者姓名： | 梁平彦 陈开英 |
| 作者单位： | 中国科学院微生物研究所, 北京 |
| 摘 要： | 自山东泰安及烟台地区的小麦全蚀病菌中提取到两种类型颗粒,一类是球形至多边形的病毒颗粒,直径在22~45毫微米之间,多数为27~32毫微米;另一类是棒状颗粒,长度为40~185毫微米×16毫微米,平均108×16毫微米,提取的球形病毒具有核蛋白的典型吸收光谱,最大和最小吸收值的波长分别在260毫微米和240毫微米,A260/240=1.24,A260/280=1.62。以烟台株病毒制备的抗血清,分别与烟台及泰安株病毒在琼脂免疫双扩散中作用均产生沉淀线,表明二者具有共同的血清型。烟台株病毒经苯酚抽提后的核酸样品最大吸收波长是260毫微米,最小是230毫微米,经聚丙烯酰胺-琼脂糖凝胶电泳分离为两条迁移率相近的带,表明此病毒有两个基因片断,在分类上属于需要两个dsRNA片断才能复制的等轴dsRNA真菌病毒。其外壳蛋白经测定含分子量70,000的多肽。<>br从浙江、湖北等11个省(市)的12株全蚀病菌中均提取到了病毒,比较了各菌的菌落颜色,生长速度和病毒浓度。 |
STUDY OF MYCOVIRUS INFECTED GAEUMANNOMYCES(OPHIBOLUS) GRAMINIS |
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| Liang Pingyan,Chen Kaiying.STUDY OF MYCOVIRUS INFECTED GAEUMANNOMYCES(OPHIBOLUS) GRAMINIS[J].Acta Phytopathologica Sinica,1984,14(3):159-164. | |
| Authors: | Liang Pingyan Chen Kaiying |
| Institution: | Instiute of Microbiology, Academia Sinica, Beijing |
| Abstract: | Two types of virus like particles have been extracted from mycelium of Gaeumannomyces(Ophiobolus) graminis isolated from infected roots of wheat incities Yantai and Taian,Shandong Province.One is spherical to polyhedral 22-45 nm and predominal 27-32 nm in diameter,the other is rod-shaped about 16 nm in width and 40-108-185 nm in length with a broad central canal.The rod-shaped particles were seldom found in extract. The spherical virus particle preparation exhibited an ultraviolet absorption spectrum typical of nucleoprotein with λ maximum at 260 nm and λ minimum at 240 nm,A260/280=1.62,A260/240=1.24.When the spherical particles of Yantai or Taian isolates were placed in 0.8% agar immunodiffusion plates and tested against antiserum prepared with virus of Yantai isolate,specific precipitin lines appeared.The result indicated the same serotype between the two isolates. The dsRNA preparation extracted from virus of Yantai isolate had a maximum absorbance at 260 nm and minimum absorbance at 230 nm.When preparations were subjected to electrophoresis in 2.4% polyacrylamide agarose dis gel,two clear bands of closely similar mobility were obtained,thus showing that the Yantai isolate contained two genome and taxonomically belonged to the possible family i.e.isometric dsRNA mycoviruses requiring two RNA segments for replication.The virus contained mainly one capsid polypeptide with a molecular weight of 70,000 dalton. Extracts of 12 isolates collected from nine provinces(Zhejian,Hubei,Neimenggu,Heilongjiang,Jiangsu,Hebei,Shanxi,Shandong,Qinghai) were examined under the electron microscope,showing that all isolates contained spherical virus particles.The colony colourations,growth rates and virus concentration of all these isolates were observed. |
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