| 土壤链霉菌CaiF1抗菌物质的分离纯化及活性研究(英文) |
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| 引用本文： | 杨辉,彭桂香,曾建民,谭志远.土壤链霉菌CaiF1抗菌物质的分离纯化及活性研究(英文)[J].农业科学与技术,2012(7):1460-1464. |
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| 作者姓名： | 杨辉 彭桂香 曾建民 谭志远 |
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| 作者单位： | 华南农业大学农学院 |
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| 基金项目： | 高等学校博士学科点专项科研基金(20094404110006);国家重点基础研究发展计划(2010CB126502);广东省科技厅项目(2010B0602-00017,2010B090400450)资助~~ |
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| 摘 要： | 目的]从土壤链霉菌CaiF1发酵液中分离纯化抗菌物质,并研究该物质的活性。方法]采用乙酸乙酯萃取,大孔吸附树脂吸附,硅胶层析和制备型高效液相色谱(HPLC)等分离技术纯化抗菌物质,以金黄色葡萄球菌、白粉病菌为指示菌进行活性研究。结果]土壤链霉菌CaiF1发酵液中抗菌物质得到分离纯化;该物质在pH2.0-pH10.0、100℃和紫外照射24h条件下抑菌活性几乎不变,对白粉病的防治效果达69.7%。结论]分离纯化的土壤链霉菌CaiF1抗菌物质具有很好的稳定性,为其结构鉴定和开发应用提供了理论依据。
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| 关 键 词： | 土壤链霉菌 抗菌物质 分离纯化 稳定性 |
Purification and Activity of Antibacterial Substances Derived from Soil Streptomyces sp.CaiF1 |
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| Hui YANG, Guixiang PENG, Jianmin ZENG, Zhiyuan TAN.Purification and Activity of Antibacterial Substances Derived from Soil Streptomyces sp.CaiF1[J].Agricultural Science ＆ Technology,2012(7):1460-1464. |
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| Authors: | Hui YANG Guixiang PENG Jianmin ZENG Zhiyuan TAN |
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| Institution: | College of Agriculture, South China Agricultural University, Guangzhou 510642, China |
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| Abstract: | Objective] This study aimed to separate and purify antibacterial substances from soil Streptomyces sp. CaiF1, and to explore the activities of this substance. Method] The antibacterial substances were separated and purified by Ethyl acetate extraction, macroporous adsorptive resin, silica gel chromatography and preparative high performance liquid chromatography (HPLC), and powdery mildew were taken as the indicating bacterial to study their activities. Result] Antibacterial substances were purified and the stability analysis of the extracts from Streptomyces CaiF1 fermentation broth showed very stable at pH 2.0-pH 10.0, 100 ℃ and changed very little under UV treatment for 24 h. Inhibition rate of powdery mildew was 69.7%. Conclusion] The purified antibacterial substances showed good stability, which provided theoretical foundation for their structural identifications and future applications. |
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| Keywords: | Soil Streptomyces sp Antibacterial substances Separation and purification Stability |
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