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| 水稻谷蛋白GluB-4基因启动子克隆及载体构建 | |
| 引用本文： | 王伟权,杨翠平,王景雪,梁超,刘莉.水稻谷蛋白GluB-4基因启动子克隆及载体构建[J].华北农学报,2007,22(2):22-25. |
| 作者姓名： | 王伟权 杨翠平 王景雪 梁超 刘莉 |
| 作者单位： | 1. 广州医学院第一附属医院呼吸疾病研究所,广东,广州,510120 2. 中山大学生命科学学院,有害生物控制及资源利用国家重点实验室,广东,广州,510275 3. 山西大学生命科学与技术学院,山西,太原,030006 |
| 摘 要： | GluB-4是水稻种子谷蛋白的编码基因,在种子成熟过程中,由GluB-4启动子调控,在胚乳中特异地表达。以水稻基因组DNA为模板,通过PCR扩增技术得到GluB-4启动子片段,序列分析结果表明:获得的启动子片段的大小为1 560 bp,与已报道的该启动子序列相比较,其核苷酸序列同源性为99.8%。该启动子区域含有TATA-box,CAAT-box,GCN4基序,Skn-1基序等胚乳特异表达启动子所必需的正调控元件,同时还含有高水平转录调控因子5UTR Py-rich stretch序列。利用该启动子构建了植物种子特异表达载体pCGB4P,为进一步的研究工作奠定了基础。 |
| 关 键 词： | 水稻 GluB-4基因 启动子 谷蛋白 |
| 文章编号： | 1000-7091（2007）02-0022-04 |
| 修稿时间： | 2007-02-07 |
Cloning and Constructing of the GluB-4 Promoter from Rice |
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| WANG Wei-quan,YANG Cui-ping,WANG Jing-xue,LIANG Chao,LIU Li.Cloning and Constructing of the GluB-4 Promoter from Rice[J].Acta Agriculturae Boreali-Sinica,2007,22(2):22-25. | |
| Authors: | WANG Wei-quan YANG Cui-ping WANG Jing-xue LIANG Chao LIU Li |
| Institution: | 1. Guangzhou Institute of Respiratory Diseases, Department of Respiratory Diseases, First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, China; 2.State Key Laboratory for Biocontrol,Sun Yat-sen University, Guangzhou 510275, China;3.School of Life Science and Technology, Shanxi University, Taiyuan 030006, China |
| Abstract: | The GluB-4 promoter was amplified from Oryza sativa genome by polymerase chain reaction and cloned into pGEM-T Easy vector.Sequence analysis showed that the obtained promoter fragment 1 560 nucleotides and showed homology of 99.8% with the reported GluB-4 promoter.Also,the obtained promoter fragment includes TATA-box,CAAT-box and the two cis-regulatory elements,GCN4 motif and Skn-1 motif, which are necessary to mediate endosperm expression of the GluB-4 glutelin gene.The plant expression vector pCGB4P was constructed by the GluB-4 promoter. |
| Keywords: | Oryza sativa GluB-4 gene Promoter Glutelin |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |