| 大花蕙兰和墨兰花粉活力测定及贮藏研究 |
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| 引用本文： | 凌春英,肖恩,严冬瑾,霍丽丽,张启翔.大花蕙兰和墨兰花粉活力测定及贮藏研究[J].安徽农业科学,2010,38(5):2312-2314. |
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| 作者姓名： | 凌春英 肖恩 严冬瑾 霍丽丽 张启翔 |
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| 作者单位： | 北京林业大学园林学院,北京,100083;北京林业大学园林学院,北京,100083;国家花卉工程技术研究中心,北京100083 |
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| 基金项目： | 国家林业局引进国际先进林业科学技术重大项目(2006-4-C08);;“十一五”国家科技支撑计划课题(2006BAD01A18) |
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| 摘 要： | 目的]快速测定大花蕙兰和墨兰花粉活力,保证人工杂交育种有效开展。方法]以大花蕙兰和墨兰花粉为材料,用离体培养法和TTC染色法分别测定花粉活力,同时进行不同温度贮藏试验。结果]TTC染色法所测得的花粉活力均略高于离体培养法,TTC对花粉染色12 h即可达到较好的观察效果;-20和-80℃冷藏花粉1年后仍保持较高活力。结论]TTC法操作简便,需时短,可作为大花蕙兰和墨兰花粉活力测定便捷、有效的方法。-20℃低温、干燥条件可作为大花蕙兰和墨兰花粉保存的简易方法。
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| 关 键 词： | 花粉 离体培养法 TTC染色法 贮藏 |
Study of Pollen Viability Testing on Cymbidium hybridum and Cymbidium sinense and Their Pollen Preservation |
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| LING Chun-ying et al.Study of Pollen Viability Testing on Cymbidium hybridum and Cymbidium sinense and Their Pollen Preservation[J].Journal of Anhui Agricultural Sciences,2010,38(5):2312-2314. |
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| Authors: | LING Chun-ying |
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| Institution: | Department of Landscape Architecture;Beijing Forestry University;Beijing 100083 |
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| Abstract: | Objective] The research aimed to test the pollen viability to improve the probability of crossing.Method] The pollen of Cymbidium hybridum and Cymbidium sinense cultivars were used as materials,and two methods were compared in pollen viability testing between in vitro germination and TTC dyeing.The pollen was stored at different conditions.Result] Pollen viability by TTC was higher than that by in vitro germination,but there was no significance between them.The pollen could be at high viability after sto... |
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| Keywords: | Pollen Germination in vitro TTC dyeing Preservation |
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