| 棘豆蠕孢菌液体发酵条件优化 |
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| 引用本文： | 时东方,赵骥民,刘春明,郑梅竹,王琦.棘豆蠕孢菌液体发酵条件优化[J].上海农业学报,2009,25(4):75-77. |
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| 作者姓名： | 时东方 赵骥民 刘春明 郑梅竹 王琦 |
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| 作者单位： | 1. 长春师范学院中心实验室,长春,130032
2. 吉林农业大学,长春,130118 |
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| 基金项目： | 国家自然科学基金项目"新记录入侵杂草禾叶慈姑(Sagittaria graminea)的种群扩散机制及生态风险评估" |
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| 摘 要： | 采用液体深层发酵的方法，研究了培养条件对棘豆蠕孢菌（Embellisia oxytropis）菌丝体生长的影响，并采用L9（3^4）正交试验优化液体发酵培养基组成。结果表明，试验范围内棘豆蠕孢菌液体发酵培养的最佳条件为：培养基初始pH6．5、培养温度24℃、装液量32％、接种量12％，在此条件下100mL发酵液的菌丝体产量为0．573g；优化培养基配方为：玉米粉5％、马铃薯20％、磷酸二氢钾0．1％、硫酸镁0．08％。菌种扩繁次数对液体发酵的影响较大，第3代开始生物量显著下降。
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| 关 键 词： | 植物内生菌 棘豆蠕孢菌 深层培养 菌丝体 |
Optimization of submerged-culture conditions of Embellisia oxytropis |
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| SHI Dong-fang,ZHAO Ji-min,LIU Chun-ming,ZHENG Mei-zhu,WANG Qi.Optimization of submerged-culture conditions of Embellisia oxytropis[J].Acta Agriculturae Shanghai,2009,25(4):75-77. |
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| Authors: | SHI Dong-fang ZHAO Ji-min LIU Chun-ming ZHENG Mei-zhu WANG Qi |
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| Abstract: | The effects of submerged.culture conditions on the mycelium growth of Embellisia oxytropis were studied,and the medium composition was optimized by the L9(3~4)orthogonal experimental method.The results showed that the optimal cultural conditions were PH 6.5 at initial stage,culture temperature of 24℃,medium volume of 32%flask capacity and pitching rate of 12%(v/v),in which the mycelium yield of 100 mL fermented broth was 0.573 g;and the optimum medium composition was 5%corn meal,20%potato,0.1%KH2P03 and 0.08%MgSO_4.The frequency of subculture had a great effect on the mycelium yield which declined significantly after the second transfer. |
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| Keywords: | Plant endophyte Embellisia oxytropis Submerged culture Mycelium |
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