| 基于农艺性状和AFLP位点的菜用大豆遗传多样性研究 |
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| 引用本文： | 李之国,李喜焕,张彩英,马峙英.基于农艺性状和AFLP位点的菜用大豆遗传多样性研究[J].中国油料作物学报,2007,29(4):391-396. |
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| 作者姓名： | 李之国 李喜焕 张彩英 马峙英 |
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| 作者单位： | 河北农业大学 / 河北省作物种质资源重点实验室，河北 保定 071001
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| 摘 要： | 基于主要农艺性状及AFLP分子标记技术，对40份入选菜用大豆品种进行遗传多样性评价，为拓宽菜用大豆种质资源基础及新品种选育提供理论依据。研究结果表明，基于农艺性状及品质性状的聚类将40份材料划分为3个类群；AFLP标记数据聚类将40个材料划分为3个AGs（AFLP-based groups）分子类群，且聚类结果与材料的地理来源无关。另外，8对AFLP引物共扩增出30条多态性条带，平均每对引物3.75个等位变异；品种间的遗传相似系数变化范围为0.3704－0.9998，平均值变化范围为0.5980－0.7924，总体平均值为0.7094，说明供试材料间的遗传相似系数较高，遗传差异较小，因此必须进一步加强菜用大豆种质资源的筛选和创新工作。
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| 关 键 词： | 菜用大豆 种质资源 农艺性状 AFLP 遗传多样性 |
| 文章编号： | 1007-9084（2007）04-0391-06 |
| 收稿时间： | 2007-07-13 |
| 修稿时间： | 2007年7月13日 |
Genetic diversity assessment of vegetable soybean varieties based on agronomic characters and AFLPs |
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| LI Zhi-guo,LI Xi-huan,ZHANG Cai-ying,MA Zhi-ying.Genetic diversity assessment of vegetable soybean varieties based on agronomic characters and AFLPs[J].Chinese Journal of Oil Crop Sciences,2007,29(4):391-396. |
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| Authors: | LI Zhi-guo LI Xi-huan ZHANG Cai-ying MA Zhi-ying |
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| Abstract: | Genetic diversity of 40 vegetable soybean varieties was evaluated based on agronomic characters and AFLPs.The results showed that the 40 varieties could be divided into 3 groups according to the agronomic traits and AFLP polymorphic loci,respectively.Thirty DNA polymorphic bands were scored from amplification of 8 AFLP primer combinations,with 3.75 markers for each primer combination in average.And the range of pair similarity coefficient was from 0.370 4 to 0.999 8;the average pair similarity coefficient was from 0.598 0 to 0.792 4 with the total average of 0.709 4.The results also showed a narrow genetic diversity among the vegetable soybean varieties tested,thus,it is necessary to expand the germplasm resource of vegetable soybean. |
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| Keywords: | AFLP |
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