| 烤烟叶片叶绿素含量的测定及其与SPAD值的关系 |
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| 引用本文： | 曾建敏,姚恒,李天福,欧阳文韬,曹忠祥.烤烟叶片叶绿素含量的测定及其与SPAD值的关系[J].分子植物育种,2009,7(1). |
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| 作者姓名： | 曾建敏 姚恒 李天福 欧阳文韬 曹忠祥 |
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| 作者单位： | 1. 云南省烟草科学研究所,玉溪,653100
2. 云南农业大学烟草学院,昆明,650201 |
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| 基金项目： | 云南省烟草专卖局(公司)科技项目 |
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| 摘 要： | 实时监测烤烟叶片叶绿素含量对植株氮素营养状况的跟踪和烟叶成熟的判断具有重要的指导作用.本实验选用两个烤烟品种K326和红花大金元(HD),分别在移栽后的45 d和50 d,进行不同试剂叶绿素提取效果的比较研究;并在移栽后的50 d、67 d和68 d进行了叶片叶绿素含量与SPAD值的关系研究.研究结果表明,不同提取试剂对烤烟K326和红花大金元叶片叶绿素提取进程的影响差异显著.试剂A(将体积比为2:1的丙酮与无水乙醇混合液配制成95%的水溶液)提取的叶绿素总量明显比另外3种试剂的高,提取能力强,速度快,效率高,在浸提K326叶片2 h后,浸提液的叶绿素浓度是80%的丙酮提取的2倍多.试剂A维持叶K326叶片绿素的稳定性也比其它3种试剂好,其在浸提4 d后,总叶绿素含量比1 d的还高,而浸提7 d后则稍有下降;其它3种提取试剂在浸提4 d和7 d后,均比1 d后的低,特别是95%的乙醇和80%的丙酮,下降幅度为1.24%～4.82%左右,在HD中观察到的趋势相似.在K326品种移栽后的50 d(R2=0.799**)和67 d(R2=0.866 2**)以及红花大金元移栽后的68 d(R2=0.883 4**)中均发现,叶片叶绿素含量与SPAD值存在极显著的正相关.采用SPAD仪测定时,应选择全展开叶片的中部,于主脉两侧,分别读取3个读数,并将6个读数值平均代表这个叶片的SPAD值.
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| 关 键 词： | 叶绿素含量 检测方法 SPAD值 |
Chlorophyll Content Determination and its Relationship with SPAD Readings in Flue-cured Tobacco |
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| Zeng Jianmin,Yao Heng,Li Tianfu,Ouyang Wentao,Cao Zhongxiang.Chlorophyll Content Determination and its Relationship with SPAD Readings in Flue-cured Tobacco[J].Molecular Plant Breeding,2009,7(1). |
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| Authors: | Zeng Jianmin Yao Heng Li Tianfu Ouyang Wentao Cao Zhongxiang |
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| Institution: | Zeng Jianmin 1 Yao Heng 1 Li Tianfu 1 Ouyang Wentao 2 Cao Zhongxiang 1 1 Yunnan Tobacco Research Institute,Yuxi,653100,2 College of Tobacco,Yunnan Agricultural University,Kunming,650201 |
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| Abstract: | Real time supervision of leaf chlorophyll content is necessary for better understanding the status of nitrogen nutrition and leaf maturity in flue-cured tobacco. This experiment was conducted in 2008 crop season at Yanhe station of Yunnan Tobacco Research Institute in Yuxi. In order to seek the best agent for plant chlorophyll extraction and determinate the relationship between chlorophyll content and SPAD readings, two varieties, K326 and Honghuadajinyuan (HD), were used in this study. Significant differen... |
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| Keywords: | Chlorophyll content Measurements SPAD readings |
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