首页 | 本学科首页   官方微博 | 高级检索  
     检索      

胡萝卜素漂白法快速筛选耐储藏水稻品种
引用本文:沈文飚,俞伟伟,汪仁,江玲,翟虎渠,程式华,万建民.胡萝卜素漂白法快速筛选耐储藏水稻品种[J].中国水稻科学,2003,17(4):387-389.
作者姓名:沈文飚  俞伟伟  汪仁  江玲  翟虎渠  程式华  万建民
作者单位:1.南京农业大学 作物遗传与种质创新国家重点实验室, 江苏省植物基因工程研究中心, 江苏 南京 210095; 2中国农业科学院, 北京 100081; 3中国水稻研究所, 浙江 杭州 310006
基金项目:国家 8 6 3计划资助项目 ( 2 0 0 1AA2 4 1 0 1 9),科技部转基因专项资助项目 (J0 0 A0 0 6 1 )
摘    要:根据水稻种胚脂氧合酶的主要同工酶LOX-3的活性缺失可以提高耐贮性的特性,研究了快速测定LOX-3酶活性以筛选耐储藏水稻品种的方法。报道了水稻种胚LOX-3具有共氧化的次生反应特性,并根据该特性建立了快速筛选其缺失体的胡萝卜素漂白法(检测时间为3 h),优化了反应体系。推测在正常氧压下水稻种胚LOX-1和LOX-2不具备或仅具备相对较弱的共氧化次生反应能力。与LOX-3缺失体的单克隆抗体技术和I2-KI比色法相比,胡萝卜漂白法具有方法简单、成本低、快速的优点。还探讨了胡萝卜素漂白法在耐储藏水稻品种改良和相关分子克隆研究中的应用前景。

关 键 词:水稻  种胚  脂氧合酶  同工酶缺失体  筛选方法  
文章编号:1001-7216(2003)04-0387-03
收稿时间:1900-01-01;

A Rapid Method for Screening Storable Rice Cultivars by Carotene Bleaching
SHEN Wen-biao ,YU Wei -wei ,WANG Ren ,JIANG Lin g ,ZHAI Hu-qu ,CHENG Shi -hua ,WAN Jian-min.A Rapid Method for Screening Storable Rice Cultivars by Carotene Bleaching[J].Chinese Journal of Rice Science,2003,17(4):387-389.
Authors:SHEN Wen-biao  YU Wei -wei  WANG Ren  JIANG Lin g  ZHAI Hu-qu  CHENG Shi -hua  WAN Jian-min
Institution:( ;1. State Key Laboratory of Crop Genetics and Germplasm Enhancement; Jiangsu Plant Gene Engineering Center; Nanjing Agricultural University; Nanjing 210095; China;2. Chinese Academy of Agricultural Sciences; Beijing 100081; China;3. China National Rice Research Institute; Hangzhou 310006)
Abstract:Based on co-oxidation secondary reaction functio n of lipoxygenase-3(LOX-3) in rice embryo, a rapid method for detecting the lack of LOX-3 in rice embryos by carotene bleaching for only three hours was developed and optimized. The method is simple, inexpensive and suitable to rapid screening storable rice cultivars compared with monoclonal antibody technique a nd indide-starch method. It was deduced that LOX-1 and LOX-2 in rice embryos probably displayed less or no co-oxidation activities under normal air conditi on in comparison with that of LOX-3. The perspective of carotene bleaching method on the usage in breeding for stora ble rice cultivars and related-cloning research was also discussed.
Keywords:rice  embryo  lipoxygenase  null of isoenzyme  scree ning methods
本文献已被 CNKI 维普 万方数据 等数据库收录!
点击此处可从《中国水稻科学》浏览原始摘要信息
点击此处可从《中国水稻科学》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号