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紫外分光光度法测定陈皮类茶饮料的酶解效果
引用本文:李循则,郭乃菲,江科新,王雪,李思洁.紫外分光光度法测定陈皮类茶饮料的酶解效果[J].安徽农业科学,2017,45(29).
作者姓名:李循则  郭乃菲  江科新  王雪  李思洁
作者单位:辽宁中医药大学药学院,辽宁大连,116600;辽宁中医药大学药学院,辽宁大连,116600;辽宁中医药大学药学院,辽宁大连,116600;辽宁中医药大学药学院,辽宁大连,116600;辽宁中医药大学药学院,辽宁大连,116600
摘    要:目的]使用紫外分光光度测定陈皮类茶饮料的酶解效果。方法]全波长扫描,确定陈皮类茶饮料样品的吸收峰,再用紫外分光光度法确定样品OD值并换算出T值,研究酶解效果。结果]方法精密度的相对标准偏差为0.086%,方法稳定性的相对标准偏差为0.210%,灵敏度比感官评价至少高125倍。结论]用紫外分光光度法研究酶解效果的方法具有较好的精密度、较高的灵敏度和较好的稳定性。

关 键 词:紫外分光光度法  陈皮  橙皮苷  准确度  精密度

Enzymes Effect of Dried Tangerine or Orange Peel Tea by Ultraviolet Spectrophotometry
Abstract:Objective] The aim was to determine enzymes effect of dried tangerine or orange peel tea by ultraviolet spectrophotometry. Method] Full wavelength scanning was used to determine the absorption peak of the dried tangerine or orange peel tea drinks, and then the sample OD value was determined by UV spectrophotometry, and figured out T value.Result]The relative standard deviation of the stability of the method was 0.086%;the RSD relative standard deviation of the stability of the method was 0.210%, and the sensitivity was higher than sensory evaluation at least 125 times. Conclusion] Effect of enzyme solution is studied with UV spectrophotometry , and the method has good precision, high sensitivity and good stability.
Keywords:Ultraviolet spectrophotometry  Dried tangerine or orange peel  Orange peel glucoside  Accuracy  Precision precision
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