| 花鲈消化系统组织学与组织化学研究 |
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| 引用本文： | 赵向炯,章利勇,朱爱意.花鲈消化系统组织学与组织化学研究[J].安徽农业科学,2017,45(30). |
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| 作者姓名： | 赵向炯 章利勇 朱爱意 |
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| 作者单位： | 浙江海洋大学,浙江舟山,316004;浙江海洋大学,浙江舟山,316004;浙江海洋大学,浙江舟山,316004 |
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| 摘 要： | 目的]探讨花鲈的生理特性。方法]用石蜡切片的方法,通过不同的染色方法对鲈鱼的消化道进行组织学和组织化学研究。结果]PAS反应显示消化道各处都有黏多糖存在;AB/PAS反应显示消化道黏膜下层含有中性黏多糖,杯状细胞含有中性、酸性黏多糖。硝酸银反应显示胃体、幽门胃黏膜下层有棕黑色颗粒,表明含钙。腾氏蓝反应显示幽门胃及结缔组织中有蓝黑色颗粒,表明含铁。HE染色显示鲈鱼消化道肌肉层发达。食道、幽门及胃含有大量杯状细胞,黏液腺发达;胃部肌肉层发达。结论]花鲈消化道结构与其肉食性密切相关。
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| 关 键 词： | 花鲈 消化道 组织化学 组织学 |
Chemical and Histochemistry Study on the Digestive System of Lateolabrax japonicus |
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| ZHAO Xiang-jiong,ZHANG Li-yong,ZHU Ai-yi.Chemical and Histochemistry Study on the Digestive System of Lateolabrax japonicus[J].Journal of Anhui Agricultural Sciences,2017,45(30). |
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| Authors: | ZHAO Xiang-jiong ZHANG Li-yong ZHU Ai-yi |
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| Abstract: | Objective] To discuss the physiological characteristics of Lateolabrax maculatus. Method] Using paraffin section method,chemi-cal and histochemistry studies were conducted on the digestive tract of L.maculatus with different staining methods. Result] PAS reaction showed that glycosaminoglycan was found in every digestive tract.AB/PAS reaction showed that neutral mucopolysaccharide was found at the submucous layer of digestive tract, neutral and acid mucopolysaccharide were found in the goblet cell.The results of silver nitrate reaction showed that there were brownish black points in stomach, pylorus andsubmucosa tissue,which meant the calcium-containing.The reaction of turpentineaction showed that blue black points were found in the pyloric, stomach, and connective tissues,which meant the iron-containing. The results of HE staining showed that the gastrointestinal muscle layer was brawny.A large number of goblet cells were found in the esopha-gus, pylorus, and stomach.Furthermore, the mucous glands developed well.And the muscle layer in stomach was brawny. Conclusion] The digestive tract structure of L.maculatus was closely correlated with its carnivorous habit. |
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| Keywords: | Lateolabrax maculatus Digestive tract Histochemistry Histology |
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