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| 水稻（脱氧）胞苷酸脱氨酶基因的表达特性分析 | |
| 引用本文： | 王学军,康厚祥,刘忠松,王国梁.水稻（脱氧）胞苷酸脱氨酶基因的表达特性分析[J].中国水稻科学,2012,26(3):261-266. |
| 作者姓名： | 王学军 康厚祥 刘忠松 王国梁 |
| 作者单位： | 1. 湖南农业大学作物基因工程湖南省重点实验室,湖南长沙,410128 2. 中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京,100193 3. 湖南农业大学作物基因工程湖南省重点实验室,湖南长沙410128;中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京100193 |
| 基金项目： | 湖南省自然科学基金资助项目(10JJ3042) |
| 摘 要： | 以水稻为材料，系统探讨了水稻中（脱氧）胞苷脱氨酶基因的组织表达特性和胁迫诱导表达特性。实时PCR结果表明，7个编码（脱氧）胞苷脱氨酶的基因在苗期水稻根、茎和叶中的表达具有组织特异性，但这些基因在水稻茎中的表达都低于根和叶中的表达；同时，这些基因胁迫诱导表达特性也不尽相同，只有Os07g0245100的表达同时受到低温、干旱、高盐和病害侵染等多种胁迫的诱导。因此，（脱氧）胞苷酸脱氨酶在水稻中可能通过调节体内嘧啶核苷的水平从而在水稻的环境胁迫应答反应中发挥重要调控作用。 |
| 关 键 词： | 胁迫 （脱氧）胞苷酸脱氨酶 基因表达 水稻 |
| 收稿时间： | 2011-09-15; |
Expression Analysis of Genes Encoding Deoxycytidine/Cytidine Deaminases in Rice |
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| WANG Xue-jun , KANG Hou-xiang , LIU Zhong-song , WANG Guo-liang.Expression Analysis of Genes Encoding Deoxycytidine/Cytidine Deaminases in Rice[J].Chinese Journal of Rice Science,2012,26(3):261-266. | |
| Authors: | WANG Xue-jun KANG Hou-xiang LIU Zhong-song WANG Guo-liang |
| Institution: | 1,2(1Hunan Provincial Key Laboratory of Crop Gene Engineering,Hunan Agricultural University,Changsha 410128,China;2State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China) |
| Abstract: | The phylogenetic relationship among the seven deoxycytidine/cytidine deaminase genes and the expression of these genes in different tissues and under different stresses were analyzed.Real-time PCR analysis showed that the seven genes had different expression characteristics in roots,stems and leaves with relative high expression levels in leaves and low expression levels in rice stems.Furthermore,the expression of most genes was stress-induced including chillness,drought,salination and disease infection,but only the expression of Os07g0245100 was induced by all four stresses.Our results demonstrated that the deoxycytidine/cytidine deaminase genes might play an important role in abiotic and biotic responses. |
| Keywords: | stress deoxycytidine/cytidine deaminase gene expression rice |
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