| 柔嫩艾美耳球虫敏感株与抗药株子孢子膜脂流动性的检测 |
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| 引用本文： | 马立农,陈杖榴.柔嫩艾美耳球虫敏感株与抗药株子孢子膜脂流动性的检测[J].西北农业学报,2010,19(8):16-21. |
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| 作者姓名： | 马立农 陈杖榴 |
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| 作者单位： | 1. 深圳职业技术学院,化生学院,广东深圳,518055
2. 华南农业大学,兽医药理研究室,广州,510642 |
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| 摘 要： | 建立了应用荧光探针1,6-二苯基-1,3,5-己三烯(DPH)测定柔嫩艾美耳球虫子孢子膜脂流动性的方法。对柔嫩艾美耳球虫(Eimeria tenella)的敏感株与各种抗药株共13个球虫株的子孢子膜脂流动性进行测定。结果表明,敏感株的膜脂流动性大,抗药株膜脂流动性小(P0.01,呈极显著相关)。由此可见,虫株对药物的敏感性可能与膜脂流动性有关,膜脂流动性的检测结果可作为判别柔嫩艾美耳球虫对离子载体类抗球虫药物敏感性或抗药性的指标之一。
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| 关 键 词： | 柔嫩艾美耳球虫 子孢子 敏感株 抗药株 膜脂流动性 |
Detection of Sporozoite Membrane Lipid Fluidity of Drug sensitive and Drug resistant Strains in Eimeria tenella |
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| MA Linong,and,CHEN Zhangliu.Detection of Sporozoite Membrane Lipid Fluidity of Drug sensitive and Drug resistant Strains in Eimeria tenella[J].Acta Agriculturae Boreali-occidentalis Sinica,2010,19(8):16-21. |
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| Authors: | MA Linong and CHEN Zhangliu |
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| Institution: | MA Linong1 and CHEN Zhangliu2(1.School of Applied Chemistry and Biological Technology,Shenzhen Polytechnic,Shenzhen Guangdong 518055,China,2.Veterinary Pharmacology Laboratory,South China Agricultural University,Guangzhou 510642,China) |
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| Abstract: | A method employing fluorescent probe of 1,6-diphenyl-1,3,5-hexatriene(DPH) to detect the sporozoite membrane lipid fluidity of Eimeria tenella was established.The sporozoite membrane lipid fluidity of 13 different strains in Eimeria tenella,including a sensitive stain and 12 drug-resistant stains,was detected by means of this method.The results were as follows: the sensitive strain had high sporozoite membrane lipid fluidity,however,the drug-resistant strains were small(probability of correlation coefficien... |
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| Keywords: | Eimeria tenella Sporozoite Drug-sensitive strain Drug-resistant strain Membrane lipid fluidity |
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