| 荔枝AFLP分析体系的建立 |
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| 引用本文： | 易干军,霍合强,蔡长河.荔枝AFLP分析体系的建立[J].果树学报,2002,19(6):361-364. |
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| 作者姓名： | 易干军 霍合强 蔡长河 |
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| 作者单位： | 广东省农业科学院果树研究所,广东,广州,510640 |
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| 基金项目： | 国家自然科学基金资助项目(39700101)，广东省自然科学基金资助项目(970678)。 |
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| 摘 要： | 以39份荔枝种质资源为试材,建立起荔枝AFLP分析体系。对AFLP分析过程中的影响因子(酶切与连接、预扩增、标记)进行了研究。双酶切必须完全彻底。选出适于荔枝AFLP分析的引物组合3对,分别为EcoRIAAC+MseICTG,EcoRIAGC+MseICAT,EcoRIACC+MseICAT。应用其中2对引物(EcoRIAAC+MseICTG,EcoRIACC+MseICAT),共在106个位点扩增出条带,多态性带92个(86.7%)。对荔枝AFLP分析的影响因子进行了分析。
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| 关 键 词： | 荔枝 AFLP分析 |
| 文章编号： | 1009-9980-(2002)-01-361-04 |
| 修稿时间： | 2002年5月16日 |
Construction of AFLP Analysis System in Litchi |
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| Yi Ganjun,Huo Heqiang,and Cai Changhe.Construction of AFLP Analysis System in Litchi[J].Journal of Fruit Science,2002,19(6):361-364. |
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| Authors: | Yi Ganjun Huo Heqiang and Cai Changhe |
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| Abstract: | The AFLP(amplified fragment length polymorphism)analysis system was established with39litchi cultivars.The results of several reactions during AFLP analysis have been detected such as double enzyme restriction,pre-amplification and la beling.Dou ble enzymes restriction should be whole complete.Three pairs of primers(Eco RIAAC+Mse ICTG,Eco-RIAGC+Mse ICAT and EcoRI ACC+Mse I CAT)with powerful distinction have been selected.The AFLP fingerprintings with two pairs of primers(EcoRIAAC+Mse ICTG,EcoRI ACC+Mse I CAT)had106clear and strong amplification bands.The factors which affect AFLP analysis were discussed. |
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| Keywords: | Litchi AFLP system AFLP(amplified fragment length polymorphisms) |
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