| 铁皮石斛中3种常用农药的残留动态及最佳清除方法研究 |
| |
| 引用本文： | 姜武,吴志刚,陶正明,金传高,宋敏全.铁皮石斛中3种常用农药的残留动态及最佳清除方法研究[J].农药学学报,2017,19(3):355-365. |
| |
| 作者姓名： | 姜武 吴志刚 陶正明 金传高 宋敏全 |
| |
| 作者单位： | 1.浙江省亚热带作物研究所, 浙江 温州 325005 |
| |
| 基金项目： | 中央农业技术推广项目（财农[2016]61号）；温州市公益性农业科技项目（N20150017）；温州市高层次人才创新技术项目（温人社发[2016]51号）；温州市大荆镇富民强镇专项（2015）；乐清市科技计划项目（2016N004） |
| |
| 摘 要： | 以被苯醚甲环唑、多菌灵及烯酰吗啉污染的铁皮石斛为研究对象，采用乙腈提取，固相分散法净化，高效液相色谱-串联质谱仪分析了3种农药在铁皮石斛鲜条及栽培基质中的残留消解动态，并检测了不同水洗时间、不同溶液浓度（氯化钠和碳酸氢钠）条件下农药残留的去除情况，通过响应面方法确定了农药最佳去除方法。结果表明:3种常用农药在不同添加水平下的回收率为78.5%~106%，相对标准偏差（RSD）为0.7%~4.5%，满足农药残留检测要求；田间试验采用推荐剂量和两倍推荐剂量处理，苯醚甲环唑在铁皮石斛鲜条和基质中的半衰期分别为3.8~3.9 d和4.1~5.6 d；多菌灵的半衰期分别为2.6 d和2.8~3.1 d；烯酰吗啉的半衰期分别为2.8~2.9 d和3.6~5.1 d。响应面法结果显示:经质量分数为5.49%的氯化钠和1.27%的碳酸氢钠混合溶液浸泡5 min后再用清水冲洗45 min，残留的苯醚甲环唑去除率最高；用5.34%的氯化钠和1.22%的碳酸氢钠混合溶液浸泡5 min后再用清水冲洗45 min，多菌灵的去除率最高；而用2.07%的氯化钠和0.80%的碳酸氢钠混合溶液浸泡5 min后再用清水冲洗59 min，烯酰吗啉的去除率最高。本研究可为铁皮石斛质量安全评价体系的建立提供基础数据。
|
| 关 键 词： | 铁皮石斛 苯醚甲环唑 多菌灵 烯酰吗啉 消解动态 响应面法 |
| 收稿时间： | 2017/3/23 0:00:00 |
Residue dynamics of 3 commonly used pesticides in Dendrobium officinale and optimization of residue removing methods |
| |
| JIANG Wu,WU Zhigang,TAO Zhengming,JIN Chuangao and SONG Minquan.Residue dynamics of 3 commonly used pesticides in Dendrobium officinale and optimization of residue removing methods[J].Chinese Journal of Pesticide Science,2017,19(3):355-365. |
| |
| Authors: | JIANG Wu WU Zhigang TAO Zhengming JIN Chuangao and SONG Minquan |
| |
| Institution: | 1.Zhejiang Institute of the Subtropical Crops, Wenzhou 325005, Zhejiang Province, China2.Zhejiang Gaobizi Biological Technology Co., Ltd, Yueqing 325616, Zhejiang Province, China3.Zhejiang Tiefengtang Biological Technology Co., Ltd, Yueqing 325616, Zhejiang Province, China |
| |
| Abstract: | The residue dynamic of difenoconazole, carbendazim and dimethomorph in fresh plant of Dendrobium officinale and its growth media were investigated. Samples extracted with acetonitrile were purified by solid-phase dispersion method and analysed by HPLC-MS/MS. The response surface method was used to modify the best cleaning mode which involved the washing time and the solution concentration (NaCl and NaHCO3). The results showed that the average recoveries of 3 commonly used pesticides were 78.5%-106% and RSDs were 0.7%-4.5% at different spiking concentrations, which meets the inspection requirement of pesticide residues. When recommended dosage and double recommended dosage of pesticides were applied, the half-lives of difenoconazole, carbendazim and dimethomorph were 3.8-3.9 d, 2.6 d and 2.8-2.9 d in fresh plant and 4.1-5.6 d, 2.8-3.1 d and 3.6-5.1 d in the growth media, respectively. The optimal cleaning conditions for difenoconazole were water washing for 45 min after washing in the mixture of 5.49% NaCl and 1.27% NaHCO3 for 5 min. As for carbendazim, the conditions were water washing for 45 min after washing in the mixture of 5.34% NaCl and 1.22% NaHCO3 for 5 min. And the conditions for dimethomorph were water washing for 59 min after washing in the mixture of 2.07% NaCl and 0.80% NaHCO3 for 5 min. This study provided stable and excellent basis for the establishment of D. officinale safety assessment system. |
| |
| Keywords: | Dendrobium officinale difenoconazole carbendazim dimethomorph degradation dynamic response surface method |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《农药学学报》浏览原始摘要信息 |
| 点击此处可从《农药学学报》下载免费的PDF全文 |
|
