| 生防链霉菌F-15摇瓶发酵条件的优化 |
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| 引用本文： | 朱春玉,朱俊丰,马杰良,王艳红,任娟,刘宏生.生防链霉菌F-15摇瓶发酵条件的优化[J].植物保护,2010,36(6):58-61. |
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| 作者姓名： | 朱春玉 朱俊丰 马杰良 王艳红 任娟 刘宏生 |
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| 作者单位： | 1. 辽宁大学生命科学院,动物资源与疫病防治重点实验室,沈阳,110036
2. 黑龙江八一农垦大学生命科学技术学院,大庆,163319 |
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| 基金项目： | 辽宁省百千万人才工程项目，辽宁省教育厅重点实验室项目，辽宁大学青年科研基金 |
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| 摘 要： | 采用单因素和正交试验设计,通过摇瓶培养对链霉菌菌株F-15产生抑菌活性物质的发酵条件进行优化。单因素试验结果表明,其最佳发酵条件为:玉米粉作碳源,KNO3作氮源,初始pH为7,培养时间为5 d,250 mL的摇瓶装液量为60 mL,接种量为150μL。优化后的培养基配比为:玉米粉3.0%、KNO33.0%、MgSO40.2%、NaCl 0.2%。
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| 关 键 词： | 链霉菌 发酵条件 生物防治 |
Optimization of fermentation conditions of the antagonistic Streptomyces strain F-15 |
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| Zhu Chunyu,Zhu Junfeng,Ma Jieliang,Wang Yanhong,Ren Juan,Liu Hongsheng.Optimization of fermentation conditions of the antagonistic Streptomyces strain F-15[J].Plant Protection,2010,36(6):58-61. |
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| Authors: | Zhu Chunyu Zhu Junfeng Ma Jieliang Wang Yanhong Ren Juan Liu Hongsheng |
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| Institution: | 1. Key Laboratory of Animal Resources and Epidemic Prevention, College of Life Sciences, Liaoning University, Shenyang110036, China;2. College of Biological Science and Technology, Heilongjiang Bayi Agricultural University, Daqing163319, China |
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| Abstract: | The fermentation conditions of the antagonistic substances of the Streptomyces strain F 15 produced in shake flasks were optimized by using the method of single factor and orthogonal experimental design. The results showed that the optimal carbon and nitrogen sources were corn flour and KNO3. Initial pH 7 of medium, culture for 5 days, 60 mL liquid volume in 250 mL shake flask, and an inoculum size of 150 μL were optimal fermentation conditions. Based on orthogonal experiments, the best culture medium composition was 3.0%corn flour, 3.0% KNO3, 0.2% MgSO4 and 0.2%NaCl. |
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| Keywords: | Streptomyces fermentation condition biological control |
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