| 大豆底荚高度QTL定位及候选基因挖掘 |
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| 引用本文： | 赵圆圆,李瑞超,蒋洪蔚,王乔,谢建国,刘春燕,武小霞,陈庆山.大豆底荚高度QTL定位及候选基因挖掘[J].中国油料作物学报,2020(1):51-60. |
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| 作者姓名： | 赵圆圆 李瑞超 蒋洪蔚 王乔 谢建国 刘春燕 武小霞 陈庆山 |
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| 作者单位： | 东北农业大学农学院大豆遗传改良实验室;吉林省农业科学院 |
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| 基金项目： | 黑龙江省自然科学基金(C2018017);国家重点研发计划子项目(2016YFD0100201-21);国家自然科学基金(31771882;31971899);吉林省农业科技创新项目(C92070420);黑龙江省杰出青年自然科学基金(JC2017006;JC2016004)。 |
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| 摘 要： | 底荚高度是衡量大豆品种是否适宜机械收获的关键指标。底荚高度较低的品种在机械收割过程中可能造成植株部分被切断或漏割,引起总产量损失。因此,大豆底荚高度候选基因对大豆机械化育种至关重要。本研究利用完备区间作图法(Inclusive Composite Interval Mapping, ICIM)对208染色体片段代换系群体(chromosome segment substitution lines, CSSL)进行大豆底荚高度QTL定位,获得9个与大豆底荚高度相关的QTL,分布在8条连锁群上。结合BSA重测序结果,将与大豆底荚高度相关的QTL定位到C1连锁群上1.1Mb和L连锁群上0.05Mb的区间内,并对其进行基因注释。通过基因注释数据库和信息学分析,在两个共识QTL区间内获得5个可能与大豆底荚高度相关的候选基因。这些结果可以为大豆底荚高度QTL精细定位以及机械化优质高产大豆品种的选育提供理论依据。
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| 关 键 词： | 大豆 底荚高度 完备区间作图 BSA 候选基因挖掘 |
QTL mapping and candidate gene mining for first pod height in soybean |
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| ZHAO Yuan-yuan,LI Rui-chao,JIANG Hong-wei,WANG Qiao,XIE Jian-guo,LIU Chun-yan,WU Xiao-xia,CHEN Qing-shan.QTL mapping and candidate gene mining for first pod height in soybean[J].Chinese Journal of Oil Crop Sciences,2020(1):51-60. |
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| Authors: | ZHAO Yuan-yuan LI Rui-chao JIANG Hong-wei WANG Qiao XIE Jian-guo LIU Chun-yan WU Xiao-xia CHEN Qing-shan |
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| Institution: | (Laboratory of Soybean Genetic Improvement,Northeast Agricultural University,Harbin 150030,China;Jilin Academy of Agricultural Sciences,Changchun 130033,China) |
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| Abstract: | The first pod height is a key indicator of soybean varieties suitable for mechanical harvest.Varieties with lower first pod height may cause part of the plant to be cut or missed during mechanical harvesting,resulting in total yield loss.Therefore,the candidate genes related to the first pod high traits in soybean is very important for soy bean mechanization breeding.In this study,the Inclusive Composite Interval Mapping(ICIM)method was used to map the QTLs of soybean first pod height traits for 208 chromosome segment substitution lines(CSSL),and obtained 9 QTLs related to soybean first pods height which were distributed on 8 linkage groups.Combined with the BSA re sequencing results,QTLs related to soybean first pod height were mapped to 1.1Mb on the C1 linkage group and 0.05Mb on the L linkage group,and gene annotation was performed.Through gene annotation database and informat ics analysis,five candidate genes related to soybean first pod height were obtained in two consensus QTL intervals.These results provided theoretical basis for fine QTL mapping of soybean first pod height and selective breeding of mechanized high-quality and high-yield soybean varieties. |
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| Keywords: | soybean first pod height ICIM BSA candidate gene mining |
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