| P-loop，Bet v 1结构域的缺失及突变对GmPR10 和Gly m 4l 抑制大豆疫霉菌能力的影响 |
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| 引用本文： | 方馨,范素杰,秋义明,宋波,刘珊珊,吴俊江,张淑珍,徐鹏飞.P-loop，Bet v 1结构域的缺失及突变对GmPR10 和Gly m 4l 抑制大豆疫霉菌能力的影响[J].中国油料作物学报,2020,42(5):818. |
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| 作者姓名： | 方馨 范素杰 秋义明 宋波 刘珊珊 吴俊江 张淑珍 徐鹏飞 |
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| 作者单位： | 1. 东北农业大学大豆研究所/大豆生物学教育部重点实验室，黑龙江哈尔滨，150030；
2. 吉林农业大学农学院，吉林长春，130118；
3. 黑龙江省农业科学院大豆研究所/农业农村部大豆栽培重点实验室, 黑龙江哈尔滨，150086 |
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| 基金项目： | 国家自然科学基金（31971972，31671719） |
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| 摘 要： | 大豆疫霉根腐病是由大豆疫霉菌（Phytophthora sojae）引起的危害大豆生长的严重病害。课题组前期研
究表明具有P-loop 结构域的GmPR10（Gene Bank accession no. FJ960440)和具有P-loop、Bet v1 结构域的Gly m 4l
（Gene Bank accession no. HQ913577.1)抑制大豆疫霉菌生长，并且过表达GmPR10 和Gly m 4l 的转基因大豆植株可
以提高对大豆疫霉根腐病的抗性。为研究GmPR10 和Gly m 4l 抑菌机理，本研究利用点突变技术，获得了GmPR10
的P-loop结构域突变体（Gly48/Thr48和Gly51/Arg51）、Gly m 4l的P-loop结构域突变体（Gly49/Ile49和Lys55/Pro55）、GmPR10
和Gly m 4l的P-loop结构域以及Gly m 4l的Bet v1结构域缺失突变体，并纯化回收相应突变体蛋白，进行体外抑制
大豆疫霉菌试验。结果表明，突变或缺失P-loop，Bet v1结构域的GmPR10和Gly m 4l失去了抑制大豆疫霉菌（Race
1）生长的能力，说明P-loop、Bet v 1结构域对GmPR10和Gly m 4l行使抑菌功能至关重要。
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| 关 键 词： | 大豆疫霉菌 GmPR10 Gly m 4l P-loop结构域 Bet v 1结构域 缺失突变 |
Effects of P-loop,Bet v 1 domain deletion and mutation on ability of GmPR10 and Gly m 4l to inhibit Phytophthora sojae |
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| FANG Xin,FAN Su-jie,QIU Yi-ming,SONG Bo,LIU Shan-shan,WU Jun-jiang,ZHANG Shu-zhen,XU Peng-fei.Effects of P-loop,Bet v 1 domain deletion and mutation on ability of GmPR10 and Gly m 4l to inhibit Phytophthora sojae[J].Chinese Journal of Oil Crop Sciences,2020,42(5):818. |
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| Authors: | FANG Xin FAN Su-jie QIU Yi-ming SONG Bo LIU Shan-shan WU Jun-jiang ZHANG Shu-zhen XU Peng-fei |
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| Institution: | 1. Soybean Research Institute of Northeast Agricultural University, Key Laboratory of Soybean Biology in Chinese
Education Ministry, Harbin150030,China;
2. Agricultural College, Jilin Agricultural University, Changchun 130118, China;
3. Soybean Research Institute of Heilongjiang Academy of Agricultural Sciences, Key Laboratory of
Soybean Cultivation of Ministry of Agriculture and Rural Affairs, Harbin 150086, China |
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| Abstract: | Phytophthora root rot caused by the ommycete pathogen Phytophthora sojae is a serious disease af?
fecting soybean growth. In our previous study, it showed that GmPR10 with P-loop domain and Gly m 4l with both
p-loop and Bet v1 domains could inhibit the growth of Phytophthora sojae, and transgenic soybean plants of overex?
pressing GmPR10 and Gly m 4l could increase resistance to Phytophthora sojae. In order to further study the patho?
gen inhibition mechanism of GmPR10 and Gly m 4l, the P - loop structure domain mutant of GmPR10 (Gly48/
Thr48 and Gly51/Arg51), the P - loop structure domain mutant of Gly m 4l (Gly49/Ile49 and Lys55/Pro55), the P - loop
structure domain deletion mutant of GmPR10 and Gly m 4l，the Bet v1 structure domain deletion mutant of Gly m
4l by using the point mutation technique were obtained and the purified proteins of corresponding mutations to in?
hibit Phytophthora sojae in vitro were carried out. The results demonstrated that GmPR10 and Gly m 4l with P-loop
or Bet v1 domain mutation or deletion lost ability to inhibit the growth of Phytophthora sojae（Race 1), indicating
that P-loop and Bet v1 domain were crucial for GmPR10 and Gly m 4l to perform anti-pathogen functions. |
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| Keywords: | Phytophthora sojae GmPR10 Gly m 4l P-loop domain Bet v 1 domain deletion and mutation |
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