| 南瓜银叶突变体48a的表型特征及其遗传学分析 |
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| 引用本文： | 汪端华,杨建国,李倩,吴双花,何长征,马艳青.南瓜银叶突变体48a的表型特征及其遗传学分析[J].中国瓜菜,2020(4):12-16. |
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| 作者姓名： | 汪端华 杨建国 李倩 吴双花 何长征 马艳青 |
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| 作者单位： | 湖南农业大学园艺学院;湖南省农业科学院蔬菜研究所 |
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| 基金项目： | 湖南省重点研发计划(2018NK2024);湖南千亿蔬菜产业提质增效关键技术研究与示范(2019SC01-1);湖南省农业科技创新资金项目(2016QN27)。 |
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| 摘 要： | 南瓜银叶突变体48a是在嫩食型中国南瓜中分离筛选到的稳定遗传自交系,银色叶不仅可以作为标记性状应用到育种中,还可为南瓜抗虫、抗病、耐寒等一系列研究提供重要的材料基础。笔者对银叶突变体的表型特征及叶片的解剖结构进行鉴定分析,发现植株整体长势、熟性与野生型无明显差异;成熟叶片正面全部呈银灰色,叶绿素含量明显降低,叶片上表皮细胞与栅栏细胞间明显剥离,存在明显的空隙。利用突变体48a和野生型49a南瓜自交系构建的六世代遗传群体,调查发现F2的绿叶与银叶符合3∶1的分离比,回交群体BC1P1分离比符合1∶1,表明南瓜银色叶性状由单隐性基因控制。
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| 关 键 词： | 南瓜 银叶突变体 表型特征 遗传学分析 |
Phenotypic characteristics and genetic analysis of pumpkin (Cucurbita moschata Duch.)silver leaf mutant 48a |
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| WANG Duanghua,YANG Jianguo,LI Qian,WU Shuanghua,HE Changzheng,MAYanqing.Phenotypic characteristics and genetic analysis of pumpkin (Cucurbita moschata Duch.)silver leaf mutant 48a[J].China Cucurbits And Vegetables,2020(4):12-16. |
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| Authors: | WANG Duanghua YANG Jianguo LI Qian WU Shuanghua HE Changzheng MAYanqing |
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| Institution: | (Hunan Agricultural University,College of Horiticulture,Changsha 410125,Hunan,China;Hunan Vegetable Research Institute,Hunan Academy of Agricultural Sciences,Changsha 410128,Hunan,China) |
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| Abstract: | Pumpkin silver leaf mutant 48 a is a stable genetic inbred line isolated from tender pumpkin(Cucurbita moschata Duch.),which can be used not only as a marker trait in breeding, but also provide a series of important information such as insect resistance, disease resistance, cold tolerance. In this study, the phenotypic characteristics of silver leaf mutants and the anatomical structure of the leaves were identified, and we found that there was no significantly difference on growth and maturity between mutant 48 a and wild type, but the content of chlorophyll was significant decreased and a clear gap exsisted between the upper epidermal cells and the fence cells in mutant 48 a. From analysis of the sixth-generations group builded by mutant 48 a and wild-type, the result shows that F2 green leaves and silver leaves was 3∶1 separation ratio, and 1∶1 separation ratio between the backcross population BC1P1, indicated that silver leaf traits were controlled by single recessive gene. |
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| Keywords: | Pumpkin Silver leaf mutant Phenotypic characteristics Genetic analysis |
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