| 缺磷响应基因OsSQD2.1对水稻生长发育的影响 |
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| 引用本文： | 刘璐,许传山,孙雅菲,胡志,艾昊,刘秀丽,王小文,孙淑斌.缺磷响应基因OsSQD2.1对水稻生长发育的影响[J].中国水稻科学,2020,34(3):237-244. |
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| 作者姓名： | 刘璐 许传山 孙雅菲 胡志 艾昊 刘秀丽 王小文 孙淑斌 |
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| 作者单位： | 1.南京农业大学 资源与环境科学学院,南京 210095;2.临沂市兰山区农业局, 山东 临沂 276000;3.上海市农业科学院 生态环境与保护研究所, 上海 201403;4.南京农业大学 园艺学院 园林系,南京210095 |
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| 基金项目： | 国家自然科学基金资助项目(31672226);转基因生物新品种培育科技重大专项(2016ZX08009-003-005) |
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| 摘 要： | 【目的】旨在研究水稻缺磷响应基因OsSQD2.1对水稻生长发育的影响,从而解析其作用。【方法】通过生物信息学的方法,确定OsSQD2.1基因及蛋白结构并分析OsSQD2.1启动子上的顺式作用元件;通过荧光定量PCR检测,研究不同缺素条件下OsSQD2.1的表达;测定T-DNA插入突变体和沉默干涉材料不同时期表型、磷含量和净光合速率,研究OsSQD2.1对转基因植株生长发育及光合作用的影响。【结果】OsSQD2.1基因编码区全长为3548 bp,位于第1染色体上,具有11个外显子和10个内含子,OsSQD2.1属于糖基转移酶家族;OsSQD2.1启动子上含有多个缺磷响应顺式作用元件;OsSQD2.1受缺磷诱导,缺硫抑制。营养生长期突变体或沉默材料地上部长度和主根长均显著低于野生型。生殖生长期,突变体或沉默材料株高和千粒重显著低于野生型,结实率无显著差异。OsSQD2.1的突变与沉默增加正常供磷时叶片中的总磷,在缺磷条件时野生型相比无明显差异;此外,苗期和成熟期突变体的净光合速率显著低于野生型,初步推测OsSQD2.1影响水稻净光合速率。【结论】OsSQD2.1受缺磷响应,并影响水稻生长发育。
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| 关 键 词： | 磷 缺磷响应 缺磷 水稻 生长发育 |
| 收稿时间： | 2019-06-28 |
| 修稿时间： | 2019-10-22 |
Effects of Phosphate Starvation Responses Gene OsSQD2.1 on Growth in Rice |
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| Lu LIU,Chuanshan XU,Yafei SUN,Zhi HU,Hao AI,Xiuli LIU,Xiaowen WANG,Shubin SUN.Effects of Phosphate Starvation Responses Gene OsSQD2.1 on Growth in Rice[J].Chinese Journal of Rice Science,2020,34(3):237-244. |
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| Authors: | Lu LIU Chuanshan XU Yafei SUN Zhi HU Hao AI Xiuli LIU Xiaowen WANG Shubin SUN |
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| Institution: | 1.College of Resources and Environmental Science, Nanjing Agricultural University, Nanjing 210095, China;2.Agricultural Bureau of Lanshan District, Linyi City, Linyi 276000, China;3.Eco-Environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China;4.Landscape Architecture Department, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China |
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| Abstract: | 【Objective】This work aims at confirming the effects of gene OsSQD2.1, involved in phosphate starvation responses on rice growth so as to reveal its function. 【Method】The bioinformatics method was used to determine the OsSQD2.1 gene and its protein structure and the cis-acting elements on the OsSQD2.1 promoter. The expression of OsSQD2.1 under different deficiency conditions was measured by real-time PCR. For an insight into the effects of OsSQD2.1 on the growth and photosynthesis of transgenic plants, we determined the phenotype, phosphorus content and net photosynthetic rate of DNA insertion mutants and silencing interfering materials at different growth stages. 【Result】The coding region of the OsSQD2.1 gene is 3548 bp in length and it is located on chromosome 1, with 11 exons and 10 introns. OsSQD2.1 belongs to the glycosyltransferase family; OsSQD2.1 promoter contains multiple reported cis-acting elements responsive to phosphorus deficiency; OsSQD2.1 was induced by phosphorus deficiency and inhibited by sulfur deficiency. Compared with the wild type, the shoot length and primary root length during the vegetative growth period in the mutant or silencing materials were significantly lower than the wild type. During the reproductive growth, the plant height as well as 1000-grain weight of the mutant or silencing material was significantly lower than that of wild type, and there was no significant difference in seed setting rate; The total phosphorus contents in the leaves were not significantly different in the wild type under the phosphorus deficiency condition. In addition, the net photosynthetic rate of the seedling and mature mutants was significantly lower than that of the wild type, and it was presumed that OsSQD2.1 affected the net photosynthetic rate of rice. 【Conclusion】The results confirm that OsSQD2.1 is phosphorus deficiency-responsive and affects rice growth. |
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| Keywords: | phosphate phosphate starvation response phosphorus deficiency rice development |
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