| 春兰菌根真菌的分离及培养特征研究 |
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| 引用本文： | 陶诗,刘一令,黄小香,徐强,邓娜,黎勇,张霞.春兰菌根真菌的分离及培养特征研究[J].北方园艺,2010(16):108-111. |
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| 作者姓名： | 陶诗 刘一令 黄小香 徐强 邓娜 黎勇 张霞 |
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| 作者单位： | 内江师范学院,化学与生命科学学院,四川省特色农业资源研究与利用重点实验室,四川,内江,641112;内江市第二中学,四川,内江641000 |
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| 摘 要： | 采用改良PDA分离培养基,通过切片法从春兰(Cymbidium goeringii Rchb.f.)菌根中分离内生真菌,并用打孔法分离得到纯化菌株。初步鉴定为6株内生真菌(LC-1、LC-2、LC-3、LC-4、LC-5、LC-6)。结果表明:切片分离时的表面消毒条件和方法以及分离培养基的选用,均直接影响菌根真菌的分离纯化结果,及分离得到的内生菌的多样性。以75%酒精处理30 s+0.1%升汞处理5 min进行表面消毒,效果稳定,配合改良的PDA培养基分离内生菌,可以分离出较多的内生菌种类。
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| 关 键 词： | 春兰 菌根 内生真菌 分离 培养特性 |
Isolation and Culture Features of Cymbidium goeringii Mycorrhizal Fungi |
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| TAO Shi,LIU Yi-ling,HUANG Xiao-xiang,XU Qiang,DENG Na,LI Yong.Isolation and Culture Features of Cymbidium goeringii Mycorrhizal Fungi[J].Northern Horticulture,2010(16):108-111. |
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| Authors: | TAO Shi LIU Yi-ling HUANG Xiao-xiang XU Qiang DENG Na LI Yong |
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| Institution: | TAO Shi1,LIU Yi-ling1,HUANG Xiao-xiang1,XU Qiang,DENG Na2,LI Yong1(1.Chemistry and Life Science College,Neijiang Teachers College,Characteristic of Sichuan Agricultural Resources,Research and Use of the Laboratory,Neijiang,Sichuan 641112,2.The Second School in Neijiang,Sichuan 641000) |
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| Abstract: | Six Mycorrhizal endophyte strains was isolated from Cymbidium goeringii,the modified PDA medium and punching method was used to get purcultures.Isolated strains was named as LC-1,LC-2,LC-3,LC-4,LC-5,LC-6 respectively.The results showed that slice separation conditions,methods of surface disinfection,as well as the selection of media for isolation had a direct impact on the results of mycorrhizal fungi separation.For surface disinfection,75%ethanol,30 seconds+0.1%mercuric chloride,five minutes,show stable ef... |
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| Keywords: | Cymbidium goeringii endotropic mycorrhizae mycorrhizal fungus isolation culture features |
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