| 单抗夹心ELISA检测免疫鸡群新城疫病毒 |
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| 引用本文： | 蒋凤英,胡建华,倪建平,李春华,孙凤萍,王英,顾彩菊,周震祥,黄启忠,高骏.单抗夹心ELISA检测免疫鸡群新城疫病毒[J].上海交通大学学报(农业科学版),2004,22(3):286-289. |
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| 作者姓名： | 蒋凤英 胡建华 倪建平 李春华 孙凤萍 王英 顾彩菊 周震祥 黄启忠 高骏 |
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| 作者单位： | 上海市农业科学院,畜牧兽医研究所,上海,201106 |
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| 基金项目： | 上海市农业科学院青年基金项目(未编号) |
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| 摘 要： | 用SPF鸡胚将送检的免疫鸡群泄殖腔棉拭样品盲传一代，再用新城疫单抗夹心ELISA对F1代尿囊液进行新城疫病毒(NDV)快速检测，同时用世界动物卫生组织(OIE)推荐的NDV分离及鉴定的方法加以验证。通过对3423份样品的检测，检出阳性样品453份，阴性样品2959份，可疑样品11份，两种方法的阳性符合率为99．3％，阴性符合率为99．7％。对全部阳性样品进行病毒分离，经血凝(HA)和血凝抑制(HI)试验证明均含有新城疫病毒(NDV)，再将全部阳性样品分离物进一步作毒力鉴定，结果有强毒力株、中等毒力株及弱毒力株，因此用新城疫单抗夹心ELISA检测免疫鸡群泄殖腔棉拭样品F1代尿囊液新城疫病毒快速、准确率高，呈阳性者不全是NDV强毒力株感染，还有NDV中等毒力株或弱毒力株感染。
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| 关 键 词： | 单抗夹心ELISA 免疫 鸡 新城疫病毒 检测 |
| 文章编号： | 1671-9964(2004)03-0286-04 |
| 修稿时间： | 2004年2月22日 |
Detection of Newcastle Disease Virus in Vaccinated Chicken Flock with the Sandwich ELISA Based on Monoclonal Antibody |
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| JIANG Feng-ying,HU Jian-hu,NI Jian-ping,LI Chun-hu,SUN Feng-ping,WANG Ying,GU Cai-ju,ZHOU Zhen-xiang,HUANG Qi-zhong,Gao Jun.Detection of Newcastle Disease Virus in Vaccinated Chicken Flock with the Sandwich ELISA Based on Monoclonal Antibody[J].Journal of Shanghai Jiaotong University (Agricultural Science),2004,22(3):286-289. |
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| Authors: | JIANG Feng-ying HU Jian-hu NI Jian-ping LI Chun-hu SUN Feng-ping WANG Ying GU Cai-ju ZHOU Zhen-xiang HUANG Qi-zhong Gao Jun |
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| Abstract: | Cloacal swab specimens from vaccinated chicken flock were passed o nc e in 10-day-old SPF embryonated chicken eggs. The allantoic fluid was harveste d from 24h to 96h post-innoculation. The sandwich ELISA based on monoclonal ant ibodies was applied to detect Newcastle disease virus using the allantoic fluid harvested. Traditional diagnostic methods recommended by OIE confirmed the NDV v iruses. The results showed that there are 453 positive samples, 2959 negative sa mples and 11 suspicious samples of total 3423 samples detected. The coincidence rate of positive samples is 99.3%and that of negative ones is 99.7%. Further v irulence identification indicated that the velogenic, mesogenic or lentogenic ND V strains existed in these positive samples. So this ELISA method to detect of N ewcastle disease virus is rapid and correct. Positive specimens detected are not all infected by virulent viruses, it might be infected by mesogenic or lentogen ic NDV strains. |
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| Keywords: | Newcastle disease virus ELISA virulence |
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