| 大白菜‘津育60’遗传转化前后组织培养条件的优化 |
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| 引用本文： | 张,蕊 张爱红 常安涛 曹家树 李翠凤.大白菜‘津育60’遗传转化前后组织培养条件的优化[J].中国农学通报,2011,27(4):165-168. |
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| 作者姓名： | 张 蕊 张爱红 常安涛 曹家树 李翠凤 |
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| 作者单位： | 1. 南开大学生物化学与分子生物学系,天津,300071
2. 浙江大学蔬菜研究所,杭州,310029 |
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| 基金项目： | 国家自然科学基金，天津市自然科学基金 |
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| 摘 要： | 为大白菜的遗传转化研究建立较高频率的组织再生体系，选择白菜子叶-子叶柄作为外植体，通过正交试验优化了农杆菌侵染前后的组织培养条件。结果显示：侵染前的最佳分化培养基组合为MS培养基+3 mg/L 6-BA+0.3 mg/L NAA+4 mg/L AgNO3+2%蔗糖+0.8%琼脂，侵染后的最佳分化培养基组合为MS培养基+3 mg/L 6-BA+0.01 mg/L NAA+4 mg/L AgNO3+2%蔗糖+0.8%琼脂。6-BA与NAA的浓度比由侵染前的3:0.3调整为3:0.01。
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| 关 键 词： | 小麦 小麦 NaCl胁迫 种子萌发 幼苗生长 |
| 收稿时间： | 7/6/2010 12:00:00 AM |
| 修稿时间： | 2010/9/21 0:00:00 |
The Screening of Tissue Culture Medium for Brassica compestris L. ssp. pekinensis Olsson cv. ‘Jinyu60’ |
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| Zhang Rui,Zhang Aihon,Chang Antao,Cao Jiashu,Li Cuifeng.The Screening of Tissue Culture Medium for Brassica compestris L. ssp. pekinensis Olsson cv. ‘Jinyu60’[J].Chinese Agricultural Science Bulletin,2011,27(4):165-168. |
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| Authors: | Zhang Rui Zhang Aihon Chang Antao Cao Jiashu Li Cuifeng |
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| Institution: | Zhang Rui1,Zhang Aihong2,Chang Antao1,Cao Jiashu2,Li Cuifeng1(1Department of Biochemistry and Molecular Biology,Nankai University,Tianjin 300071,2Institute of Vegetable Science,Zhejiang University,Hangzhou 310029) |
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| Abstract: | To study the genetic transformation of Chinese cabbage-pe-tsai（Brassica compestris L.ssp.pekinensis Olsson‘Jinyu60’）,its tissue culture system was established.Cotyledons with petiole were used as explants,and the culture conditions were optimized by an orthogonal design.The results showed that,before and after genetic transformation,the optimum organization culture medium combination for‘Jinyu60’was：MS＋3 mg/L 6-BA＋0.3 mg/L NAA＋4 mg/L AgNO3＋2%sucrose＋0.8%agar and MS＋3 mg/L 6-BA＋0.01 mg/LNAA＋4 mg/L AgNO3＋2%sucrose＋0.8%agar,respectively.The concentration rate between 6-BA and NAA was 3：0.01. |
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| Keywords: | Chinese cabbage-pe-tsai tissue culture genetic transformation orthogonal design |
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