| 一株产胶原酶海洋微生物发酵条件的研究 |
| |
| 引用本文： | 白杨,钱斯日古楞,王红英.一株产胶原酶海洋微生物发酵条件的研究[J].中国农学通报,2011,27(4):321-325. |
| |
| 作者姓名： | 白杨 钱斯日古楞 王红英 |
| |
| 作者单位： | 大连工业大学生物工程学院,辽宁,大连,116034 |
| |
| 摘 要： | 于海洋污泥中筛选得到的Aeromonas sp.F3所产的胞外酶对胶原蛋白有水解作用，采用单因素试验法对Aeromonas sp.F3的发酵条件及培养基组分进行全面优化，提高该胶原酶生产菌的产酶效率。经优化试验结果表明，该菌株最佳发酵条件为：培养时间24 h，温度40℃，初始pH为8，装瓶量80~100 mL/250 mL；培养基组分为：葡萄糖3 g/L，酵母浸粉9 g/L，NaCl 5 g/L，MnSO4 10 mg/L。经优化试验明显提升了该菌株的产酶能力。
|
| 关 键 词： | 胶原酶 发酵条件 优化 酶活 |
| 收稿时间： | 2010/9/15 0:00:00 |
| 修稿时间： | 2010/11/4 0:00:00 |
Fermentation Conditions of a Collagenase-Producing Marine Microorganism |
| |
| Bai Yang,Qian Siriguleng,Wang Hongying.Fermentation Conditions of a Collagenase-Producing Marine Microorganism[J].Chinese Agricultural Science Bulletin,2011,27(4):321-325. |
| |
| Authors: | Bai Yang Qian Siriguleng Wang Hongying |
| |
| Institution: | Bai Yang,Qian Siriguleng,Wang Hongying(School of Biological Engineering,Dalian Polytechnic University,Dalian Liaoning 116034) |
| |
| Abstract: | Aeromonas sp.F3 screened from marine mud should secrete a protease to hydrolyze collagen.The fermentation of Aeromonas sp.F3 was investigated through single-factor test.The main factors included fermentation conditions and the medium.With single factor trial,the optimal culture condition was determined：40℃of fermentation temperature,pH 8 and volume 80~100 mL/250 mL for 24 h.The optimal liquid mediumwas：3 g/L glucose as carbon source,9 g/L yeast extract powder as nitrogen source,5 g/L NaCl as inorganic saltand 10 mg/L MnSO4as metal ion.Under the optimum condition,the enzyme activity increased very observably. |
| |
| Keywords: | collagenase fermentation conditions optimization enzyme activity |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国农学通报》浏览原始摘要信息 |
| 点击此处可从《中国农学通报》下载免费的PDF全文 |
