| 豌豆染色体制片技术的比较研究 |
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| 引用本文： | 杨起简,周禾,孙彦,杨华,刘玉芬.豌豆染色体制片技术的比较研究[J].北京农学院学报,2003,18(3):172-173,203. |
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| 作者姓名： | 杨起简 周禾 孙彦 杨华 刘玉芬 |
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| 作者单位： | 1. 北京农学院生物技术系,北京,102206
2. 中国农业大学草地所,北京,100094 |
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| 基金项目： | 北京市自然科学基金;6992007; |
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| 摘 要： | 采用3种不同的染色体制片技术,以豌豆(Pisum sativum)为材料,对常规压片(醋酸洋红染色)、孚尔根(Feulgen)核反应染色法和去壁低渗法(Giemsa染色)3种不同制片技术效果进行了比较,实验表明3种方法各有特点,去壁低渗法易获得分散细胞,有利于进行染色体核型分析。
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| 关 键 词： | 豌豆 制片技术 去壁低渗 |
| 文章编号： | 1002-3186(2003)03-0172-02 |
Compare on Technique of Making Chromosome in Garden Pea |
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| YANG Qi-Jian,ZHOU He,SUN Yan,LIU Yu-Fen.Compare on Technique of Making Chromosome in Garden Pea[J].Journal of Beijing Agricultural College,2003,18(3):172-173,203. |
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| Authors: | YANG Qi-Jian ZHOU He SUN Yan LIU Yu-Fen |
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| Abstract: | Three techniques were used to make chromosome of Garden Pea, and the compare amor them was done. The result shows three of them have their own characteristics,and the way of riddir cell wall and putting low osmosis with Giemsa dyeing is better, it is easy to get the scattering cell, ar suitable for chromosome karyotype of Pea. |
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| Keywords: | Garden Pea(Pisum sativum) technique of making chromosome way of ridding cell wall and putting low osmosis |
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