| 纤维素刚果红培养基筛选产纤维素酶菌株的影响因素研究 |
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| 引用本文： | 刘起丽,张建新,徐瑞富,孔凡彬,黄伟东.纤维素刚果红培养基筛选产纤维素酶菌株的影响因素研究[J].西北农业学报,2007,16(5):279-281. |
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| 作者姓名： | 刘起丽 张建新 徐瑞富 孔凡彬 黄伟东 |
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| 作者单位： | 1. 河南科技学院植保系,新乡,453003
2. 河南师范大学生命科学学院,新乡,453007 |
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| 摘 要： | 在利用纤维素刚果红鉴别培养基大量筛选纤维素分解菌的工作中,温度、培养基、pH值、接种量直接关系到筛选结果和工作效率,本研究通过比较与分析这些因素对水解圈出现的时间和生长速度的影响,摸索出既适合实验操作又经济节约的操作方案,为菌株的进一步大量筛选奠定技术基础。
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| 关 键 词： | 纤维素刚果红培养基 筛选 影响因素 |
| 文章编号： | 1004-1389（2007）05-0279-03 |
| 收稿时间： | 2006-09-24 |
| 修稿时间： | 2007-03-09 |
The Influence Aspects on Using Cellulose Culture Medium Containing Congo Red Differential Medium to Screen Microorganism Producing Cellulase |
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| LIU Qi-li,ZHANG Jian-xin,XU Rui-fu,KONG Fan-bin and HUANG Wei-dong.The Influence Aspects on Using Cellulose Culture Medium Containing Congo Red Differential Medium to Screen Microorganism Producing Cellulase[J].Acta Agriculturae Boreali-occidentalis Sinica,2007,16(5):279-281. |
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| Authors: | LIU Qi-li ZHANG Jian-xin XU Rui-fu KONG Fan-bin and HUANG Wei-dong |
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| Institution: | Department of Plant Protection, He''nan Institute of Science and Technology, Xinxiang He''nan 453003, China;College of Life Science, He''nan Normal University, Xinxiang He''nan 453007, China;Department of Plant Protection, He''nan Institute of Science and Technology, Xinxiang He''nan 453003, China;Department of Plant Protection, He''nan Institute of Science and Technology, Xinxiang He''nan 453003, China;Department of Plant Protection, He''nan Institute of Science and Technology, Xinxiang He''nan 453003, China |
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| Abstract: | In work of research on using culture medium containing cellulose and congo red differential medium for screening microorganism producing cellulase,the temperature,pH of the medium,inoculum size directly influence screening result and work efficiency.The paper analyzed influence of these aspects on appearance time of hydrolyze circle and growth speed,find out a suitable and economizing operation for lab,which establish a technology base for screening more microorganism. |
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| Keywords: | Cellulose and congo red differential medium Screening Influence aspects |
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