| 香蕉枯萎病生防细菌ZJ6-6的盆栽防治效果及其生防基因分析 |
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| 引用本文： | 谢晚彬,李华平.香蕉枯萎病生防细菌ZJ6-6的盆栽防治效果及其生防基因分析[J].中国果业信息,2018,47(6). |
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| 作者姓名： | 谢晚彬 李华平 |
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| 作者单位： | 华南农业大学亚热带农业生物资源保护与利用国家重点实验室/广东省微生物信号与作物病害防控重点实验室/农学院,华南农业大学亚热带农业生物资源保护与利用国家重点实验室/广东省微生物信号与作物病害防控重点实验室/农学院 |
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| 基金项目： | 国家香蕉产业技术体系专项（CAR-32-05） |
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| 摘 要： | 通过病原菌和生防菌人工接种方法测定生防细菌ZJ6-6对香蕉枯萎病的盆栽防治效果，扩增和序列分析ZJ6-6的生防基因。结果表明：ZJ6-6处理香蕉的萎缩指数和导管褪色指数比对照香蕉分别降低40.5%和43.9%，其对香蕉枯萎病具有显著防治效果；ZJ6-6至少含有6个生防基因ituD、lpa-14、bmyB、fenD、srfAA、srfAB，理论上具有合成脂肽抗生素的能力，这可能是其能防治香蕉枯萎病的原因。本研究结果对ZJ6-6合成分泌的抗菌物质鉴定及其生防机理研究具有重要意义。
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| 关 键 词： | 香蕉 枯萎病 生物防治 解淀粉芽孢杆菌 生防基因 |
| 收稿时间： | 2018/5/18 0:00:00 |
| 修稿时间： | 2018/7/13 0:00:00 |
Pot Effect of Biocontrol Bacterium ZJ6-6 against Banana Fusarium Wilt and its Biocontrol Genes |
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| LI Huaping.Pot Effect of Biocontrol Bacterium ZJ6-6 against Banana Fusarium Wilt and its Biocontrol Genes[J].China Fruit News,2018,47(6). |
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| Authors: | LI Huaping |
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| Institution: | State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources/ Guangdong Province Key Laboratory of Microbial Signals and Disease Control/ College of Agriculture,South China Agricultural University,Guangzhou |
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| Abstract: | Pot effect of biocontrol bacterium ZJ6-6 against banana Fusarium wilt was tested by inoculating with pathogen and ZJ6-6. Putative biocontrol genes of ZJ6-6 were identified by PCR amplification and sequence alignment of PCR products. Results showed that wilt index and vascular discoloration index of banana plants treated by ZJ6-6 decreased by 40.5% and 43.9% in contrast to those of control banana plants, respectively. ZJ6-6 could significantly reduce banana Fusarium wilt. Results showed that ZJ6-6 possesses biocontrol genes ituD, lpa-14, bmyB, fenD, srfAA and srfAB. Therefore, ZJ6-6 can theoretically synthesize lipopeptides, which may be the reason why ZJ6-6 can control banana Fusarium wilt. This research result helps to identify antagonistic substances produced by ZJ6-6 and to elucidate its control mechanisms. |
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| Keywords: | banana Fusarium wilt biocontrol Bacillus amyloliquefaciens biocontrol gene |
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