| 转基因鲤鲫杂交(F1)回交三倍体子代红细胞大小与DNA含量测定 |
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| 引用本文： | 闫学春,梁利群,贾智英,曹鼎臣,孙效文.转基因鲤鲫杂交(F1)回交三倍体子代红细胞大小与DNA含量测定[J].东北林业大学学报,2004,32(4):50-52. |
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| 作者姓名： | 闫学春 梁利群 贾智英 曹鼎臣 孙效文 |
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| 作者单位： | 中国水产科学研究院黑龙江研究所,哈尔滨,150070;大连水产学院;黑龙江水产研究所 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，863-101-05-02-01， |
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| 摘 要： | 通过细胞遗传学方法对转基因鲤鲫杂交(F1)回交三倍体子代鱼进行了红细胞及其核大小的测量和DNA含量的测定，结果为：采用血涂片法测量鲤鲫杂交回交子代红细胞面积和体积分别是二倍体红鲫鱼的1．43倍和1．57倍，接近三倍体的理论预期值1．5倍；通过流式细胞仪对鲤鲫杂交子代细胞和二倍体细胞进行相对DNA含量测定，结果为：在所测的12尾鱼中，有9尾鲤鲫杂交回交子代鱼的DNA含量是对照鱼的1．5倍。占总鱼数的75％，在2～3倍体之间非常接近三倍体(非整倍三倍体)的鱼有3尾，占总鱼数的25％，本结果与其细胞染色体核型分析结果基本一致。
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| 关 键 词： | 转基因鲤鲫杂交 回交子代 红细胞 DNA含量 |
The Measurements of RBC Size and DNA Content in the Backcross Triploidy Progenies of F1 Hybrids Produced by Transgenic Common Carp and Transgenic Crucian Carp |
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| Yan Xuechun,Liang Liqun.The Measurements of RBC Size and DNA Content in the Backcross Triploidy Progenies of F1 Hybrids Produced by Transgenic Common Carp and Transgenic Crucian Carp[J].Journal of Northeast Forestry University,2004,32(4):50-52. |
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| Authors: | Yan Xuechun Liang Liqun |
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| Abstract: | A study was conducted to determine red blood cell (RBC) and its nucleus size as well as DNA content in backcross progenies of hybrids produced by transgenic common carp and transgenic crucian carp by using cytogenetic methods. DNA content of backcross progenies and diploids were measured by the flow cytometry. The results show that the area and volume of RBC in backcross progenies are very close to 1.5 times as much as the theoretical expecting value of the triploids by using blood-painting method, and they are 1.43 and 1.57 times as large as those of RBC in diploids respectively. Among 12 measured progenies, the DNA contents in 9 progenies are 1.5 times as much as that of the control group, accounting for 75% of the total progenies. 3 progenies are very near to triploid (refer to as non-integral triploid), accounting for 25% of the total ones. To sum up, the results obtained are consistent with those of karyotypic analysis of cell chromosome. |
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| Keywords: | Transgenic hybrid of common carp cross crucian carp Backcross progeny Red blood cell DNA content |
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