| 茶树DNA去甲基化酶基因的全基因组鉴定及表达分析 |
| |
| 引用本文： | 陈瑶,张伟富,任恒泽,熊飞,张豪杰,姚利娜,刘莹,王璐,王新超,杨亚军,郝心愿.茶树DNA去甲基化酶基因的全基因组鉴定及表达分析[J].茶叶科学,2021,41(1):28-39. |
| |
| 作者姓名： | 陈瑶 张伟富 任恒泽 熊飞 张豪杰 姚利娜 刘莹 王璐 王新超 杨亚军 郝心愿 |
| |
| 作者单位： | 中国农业科学院茶叶研究所/国家茶树改良中心/农业农村部茶树生物学与资源利用重点实验室,浙江 杭州 310008;中国农业科学院茶叶研究所/国家茶树改良中心/农业农村部茶树生物学与资源利用重点实验室,浙江 杭州 310008;中国农业科学院茶叶研究所/国家茶树改良中心/农业农村部茶树生物学与资源利用重点实验室,浙江 杭州 310008;中国农业科学院茶叶研究所/国家茶树改良中心/农业农村部茶树生物学与资源利用重点实验室,浙江 杭州 310008;中国农业科学院茶叶研究所/国家茶树改良中心/农业农村部茶树生物学与资源利用重点实验室,浙江 杭州 310008;中国农业科学院茶叶研究所/国家茶树改良中心/农业农村部茶树生物学与资源利用重点实验室,浙江 杭州 310008;中国农业科学院茶叶研究所/国家茶树改良中心/农业农村部茶树生物学与资源利用重点实验室,浙江 杭州 310008;中国农业科学院茶叶研究所/国家茶树改良中心/农业农村部茶树生物学与资源利用重点实验室,浙江 杭州 310008;中国农业科学院茶叶研究所/国家茶树改良中心/农业农村部茶树生物学与资源利用重点实验室,浙江 杭州 310008;中国农业科学院茶叶研究所/国家茶树改良中心/农业农村部茶树生物学与资源利用重点实验室,浙江 杭州 310008;中国农业科学院茶叶研究所/国家茶树改良中心/农业农村部茶树生物学与资源利用重点实验室,浙江 杭州 310008 |
| |
| 摘 要： | DNA去甲基化酶(dMTase)是主动去甲基化过程中具有糖基化酶和脱嘌呤裂合酶活性的双功能酶.基于茶树基因组数据,本研究利用生物信息学方法对茶树DNA去甲基化酶(CsdMTase)编码基因进行了全面鉴定和序列分析.全基因组鉴定结果表明,舒茶早基因组中包含4个CsdMTases,系统进化分析将CsdMTases分为DME...
|
| 关 键 词： | 茶树 DNA去甲基化酶 逆境胁迫响应 生长发育 表达分析 |
| 收稿时间： | 2020-05-27 |
Genome-wide Investigation and Expression Analysis of DNA Demethylase Genes in Tea Plant(Camellia Sinensis) |
| |
| CHEN Yao,ZHANG Weifu,REN Hengze,XIONG Fei,ZHANG Haojie,YAO Lina,LIU ying,WANG Lu,WANG Xinchao,YANG Yajun,HAO Xinyuan.Genome-wide Investigation and Expression Analysis of DNA Demethylase Genes in Tea Plant(Camellia Sinensis)[J].Journal of Tea Science,2021,41(1):28-39. |
| |
| Authors: | CHEN Yao ZHANG Weifu REN Hengze XIONG Fei ZHANG Haojie YAO Lina LIU ying WANG Lu WANG Xinchao YANG Yajun HAO Xinyuan |
| |
| Institution: | Tea Research Institute of the Chinese Agriculture Science/National Center for Tea Improvement/Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, China |
| |
| Abstract: | DNA demethylase(dMTase)is a bifunctional enzyme with activities of glycosylase and apyrimidic lysase during active demethylation.Based on the available genome data of tea plant,bioinformatics analysis was used to comprehensively identify DNA demethylases(CsdMTase)in tea plant.The genome-wide identification results show that the tea cultivar Shuchazao contains 4 CsdMTases.These CsdMTases could be divided into two branches(DME and ROS)with differences in gene length and intron number by phylogenetic and gene structure analysis.Further analysis shows that the physical and chemical properties of different CsdMTase proteins are similar,which contain the typical conserved domains of ENCO3c and RRM_DME and are located in the nucleus.The promoter regions of CsdMTases contain a large number of cis-acting components related to light response,plant hormone response,stress response and growth and development.The CsdMTase genes are expressed in all detected tissues with certain tissue specificity.The expressions of CsdMTases were significantly up-regulated under biotic stresses,including Colletotrichum camelliae,Pseudopestalotiopsis camelliae-sinensis and Ectropis oblique treatments.During winter dormancy,different expression patterns of CsdMTases were detected in mature leaves and overwintering buds of different cultivars.The expressions of CsDMEa and CsDMEb at different stages of flower bud development and seed germination were significantly induced.The results show that active DNA demethylation process mediated by CsdMTases plays a crucial role in regulating the stress responses,growth and development of tea plant,providing a theoretical basis for discovering the epigenetic regulation mechanism in tea plant. |
| |
| Keywords: | tea plant DNA demethylase stress response growth and development expression analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《茶叶科学》浏览原始摘要信息 |
| 点击此处可从《茶叶科学》下载免费的PDF全文 |
|
