| 哈茨木霉的耐镉性及其对狗牙根耐镉能力的影响 |
| |
| 引用本文： | 卜和申,谢燕,徐倩,陈良,陈云,胡龙兴.哈茨木霉的耐镉性及其对狗牙根耐镉能力的影响[J].草地学报,2020,28(1):64-71. |
| |
| 作者姓名： | 卜和申 谢燕 徐倩 陈良 陈云 胡龙兴 |
| |
| 作者单位： | 1. 湖南农业大学农学院草业科学系, 湖南 长沙 410128;2. 中国科学院武汉植物园, 湖北 武汉 430074;3. 湖北师范大学生命科学学院, 湖北 黄石 435002 |
| |
| 基金项目： | 长沙市科技计划(kq1907042);湖南省自然科学基金(2018JJ3223);湖北省教育厅科学研究计划指导性项目(B2018151)共同资助 |
| |
| 摘 要： | 为明确耐镉微生物的生物学特性及其对植物耐镉性的影响，并为微生物-植物联合修复重金属污染土壤提供参考，以从湖南株洲清水塘化工厂重金属污染土壤样品筛选鉴定出来的一株耐镉性较强的真菌-哈茨木霉（Trichoderma harzianum）为试验材料，研究了其基本生物学特性和对镉胁迫下狗牙根（Cynodon dactylon）生长与生理的影响。结果表明：哈茨木霉生长的适宜pH约为7，最大生长量的培养时间约为72 h；哈茨木霉对镉离子的吸附率在镉浓度为100 mg·L-1时最高，达到63.54%；在镉浓度为0~300 mg·L-1条件下，哈茨木霉对镉离子的吸附方式主要为细胞壁吸附；在不同镉浓度下，哈茨木霉均能产生吲哚乙酸（Indole-3-acetic acid，IAA）；正常培养条件下（镉浓度为0 mg·L-1时），哈茨木霉产IAA量为30.88 μmol·L-1，镉浓度为300 mg·L-1时，产IAA量为10.46 μmol·L-1；外源添加哈茨木霉能有效缓解镉胁迫对狗牙根根长的抑制、降低丙二醛（Malondialdehyde，MDA）含量，并有效促进狗牙根对镉离子的吸收。本试验研究结果不仅为镉污染土壤的微生物修复提供重要菌株，同时提供了一种高效的微生物-植物联合修复重金属污染土壤的途径。
|
| 关 键 词： | 重金属 镉污染 耐镉微生物 吸附能力 狗牙根 |
| 收稿时间： | 2019-10-08 |
Cadmium Tolerance of Trichoderma Harzianum and Its Effect on Cadmium Tolerance of Bermudagrass |
| |
| BU He-shen,XIE Yan,XU Qian,CHEN Liang,CHEN Yun,HU Long-xing.Cadmium Tolerance of Trichoderma Harzianum and Its Effect on Cadmium Tolerance of Bermudagrass[J].Acta Agrestia Sinica,2020,28(1):64-71. |
| |
| Authors: | BU He-shen XIE Yan XU Qian CHEN Liang CHEN Yun HU Long-xing |
| |
| Institution: | 1. Pratacultural Science College of Agriculture, Hunan Agricultural University, Changsha, Hunan Province 410128, China;2. Wuhan Botanical Garden, The Chinese Academy of Sciences, Wuhan, Hubei Province 430074, China;3. College of Life Sciences, Hubei Normal University, Huangshi, Hubei Province 435002, China |
| |
| Abstract: | In order to elucidate the biological characteristics and the effect of microorganism on plants,the general biological characteristics and the effect of fungi on the growth and physiology of bermudagrass under cadmium(Cd)stress were investigated based on a Cd stress tolerant strain of fungi identified from the Cd-polluted soil around the chemical plant in Qingshuitang,Zhuzhou city,Hunan province.The results showed that the optimum pH of Trichoderma Harzianum was about 7,and the maximun growth time was about 72 h.The adsorption rate of cadmium ions by Trichoderma harzianum reached the highest value of 63.54%at the cadmium concentration of 100 mg·L-1.When the cadmium concentration was in the range of 0~300 mg·L-1,the main adsorption mode of cadmium ions in Trichoderma harzianum was cell wall adsorption.At different cadmium concentrations,Trichoderma harzianum produced indole-3-acetic acid(IAA).When the cadmium concentration was 0 mg·L-1,the content of IAA produced by Trichoderma harzianum was 30.88μmol·L-1.When the cadmium concentration was 300 mg·L-1,the content of IAA was 10.46μmol·L-1.Exogenous Trichoderma harzianum effectively alleviated the inhibition of cadmium stress on Bermudagrass root length,reduced the activity of POD and MDA content in Bermudagrass,and promoted the absorption of cadmium ions in Bermudagrass.The results not only provided an important strain for microbial remediation of cadmium-contaminated soil,but also provided an efficient microbial-plant combined way for the remediation of heavy metal pollution. |
| |
| Keywords: | Heavy metal Cadmium pollution Cadmium-resistant microorganisms Adsorption capacity Bermudagrass |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《草地学报》浏览原始摘要信息 |
| 点击此处可从《草地学报》下载免费的PDF全文 |
|
