| 鸡胰岛素样生长因子1受体基因G26336A、C111014A多态位点的检测及其与生产性状的相关性 |
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| 引用本文： | 金崇富,时凯,陈应江,陈长宽.鸡胰岛素样生长因子1受体基因G26336A、C111014A多态位点的检测及其与生产性状的相关性[J].中国畜牧兽医,2012,39(5):141-145. |
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| 作者姓名： | 金崇富 时凯 陈应江 陈长宽 |
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| 作者单位： | 江苏沿海地区农业科学研究所,江苏盐城,224000 |
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| 基金项目： | 江苏沿海所所基金课题，江苏省科技厅苏北科技发展计划—盐土农业技术集成与推广服务 |
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| 摘 要： | 根据GenBank发表的鸡胰岛素样生长因子1受体(insulin-like growth factor 1 receptor,IGF1R)基因序列针对外显子2和3设计2对引物,采用DNA池测序和PCR-SSCP技术,对鸡IGF1R基因的G26336A、C111014A位点进行多态性检测,并与鸡的生产性状进行相关性分析。结果表明,G26336A位点AA基因型个体56日龄体重显著低于BB基因型个体(P<0.05),AA和AB基因型个体在84日龄体重显著低于BB基因型个体(P<0.05)。C111014A位点CC、CD基因型个体初生重显著低于DD基因型(P<0.05),CC基因型个体在300日龄体重显著低于DD基因型(P<0.05)。研究结果初步表明,IGF1R基因可能是影响鸡生长性状的一个主效基因或是与之存在紧密遗传连锁的一个标记。
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| 关 键 词： | IGF1R基因 鸡 PCR-SSCP 连锁 |
| 收稿时间： | 2011-10-19 |
G26336A and C111014A Polymorphism of IGF1R Gene and its Association with Growth and Reproductive Performance in Chickens |
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| JIN Chong-fu
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SHI Kai
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CHEN Ying-jiang
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CHEN Chang-kuan.G26336A and C111014A Polymorphism of IGF1R Gene and its Association with Growth and Reproductive Performance in Chickens[J].China Animal Husbandry & Veterinary Medicine,2012,39(5):141-145. |
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| Authors: | JIN Chong-fu SHI Kai CHEN Ying-jiang CHEN Chang-kuan |
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| Institution: | Institute of Agricultural Sciences in Coastal Area of Jiangsu Province, Yanchen 224000, China |
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| Abstract: | According to GenBank published sequences for the chicken IGF1R gene,2 pairs of primers were designed for exons 2 and 3,the G26336A,C111014A loci polymorphism for IGF1R gene of chicken were detected by using the method of DNA sequencing and use of pool PCR-SSCP technique,and the correlation analysis of production traits of chicken were also studied.The results showed that AA genotype of G26336A sites showed a significant lower than BB genotype(P<0.05) for 56 days of age body weigh,AA and AB genotype showed a significant lower than BB genotype(P<0.05) for 84 days of age body weight.CC,CD genotypes of C111014A site for birth weight was significantly lower than the DD genotype(P<0.05),CC genotype for the 300 days of age body weight was significantly lower than DD genotype(P<0.05).The results preliminarily showed that the IGF1R gene is either a major gene that influences the body weight traits of chicken or a molecular marker in close linkage with such a gene. |
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| Keywords: | IGF1R gene chicken PCR-SSCP linkage |
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