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| 钼暴露对雌性小鼠肾脏脂质过氧化损伤及 Smac蛋白表达的影响 | |
| 引用本文： | 林霖,李勤凡,汪纪仓,王宏伟,张才,杨自军.钼暴露对雌性小鼠肾脏脂质过氧化损伤及 Smac蛋白表达的影响[J].中国畜牧兽医,2012,39(8):142-145. |
| 作者姓名： | 林霖 李勤凡 汪纪仓 王宏伟 张才 杨自军 |
| 作者单位： | 1. 西北农林科技大学动物医学院,陕西杨凌712100;河南科技大学动物科技学院,河南洛阳471003 2. 西北农林科技大学动物医学院,陕西杨凌,712100 3. 河南科技大学动物科技学院,河南洛阳,471003 |
| 基金项目： | 国家自然科学基金资助项目，河南科技大学基金资助项目 |
| 摘 要： | 试验旨在观察钼暴露对雌性小鼠肾脏脂质过氧化损伤及Smac蛋白表达的影响。选用60只35日龄健康雌性昆明小鼠,随机分成3组(对照组、补钼Ⅰ组、补钼Ⅱ组),分别饮用含钼浓度为0、200、400 mg/L的去离子水,连续染毒90 d后取样检测肾脏组织SOD活性和MDA含量,免疫组化法检测肾脏组织Smac蛋白表达。与对照组比较,补钼Ⅰ组SOD活性显著降低(P＜0.05),补钼Ⅱ组SOD活性极显著降低(P＜0.01);补钼Ⅱ组MDA含量较对照组显著升高(P＜0.05)。补钼Ⅰ组、补钼Ⅱ组小鼠肾皮质Smac蛋白表达极显著高于对照组(P＜0.01)。结果表明,钼暴露对小鼠肾脏能产生脂质过氧化损伤,钼可以通过上调线粒体促凋亡蛋白Smac蛋白表达参与肾脏的损伤过程。 |
| 关 键 词： | 钼 肾脏 脂质过氧化损伤 Smac蛋白 小鼠 |
| 收稿时间： | 2011-11-24 |
Effect of Molybdenum on the Lipid Peroxidation Injury and Smac Protein Expression of Kidney in Famale Mice |
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| LIN Lin , LI Qin-fan , WANG Ji-cang , WANG Hong-wei , ZHANG Cai , YANG Zi-jun.Effect of Molybdenum on the Lipid Peroxidation Injury and Smac Protein Expression of Kidney in Famale Mice[J].China Animal Husbandry & Veterinary Medicine,2012,39(8):142-145. | |
| Authors: | LIN Lin LI Qin-fan WANG Ji-cang WANG Hong-wei ZHANG Cai YANG Zi-jun |
| Institution: | 1. College of Veterinary Medicine,Northwest Agricultural and Forestry University, Yangling 712100, China; 2. College of Animal Science and Technology,Henan University of Science and Technology,Luoyang 471003,China |
| Abstract: | To investigate the effect of molybdenum on the lipid peroxidation injury and Smac protein expression of kidney in famale mice. Selected sixty 35-day-old female Kunming mice were randomly divided into 3 groups (control group, molybdenum groupⅠ, molybdenum groupⅡ). Three groups were treated with drinking water added with 0, 200, 400 mg/L molybdenum (Mo6+), respectively. On the 90th days of experiment, the SOD activity and MDA contends were measured, the expression of Smac protein were examined immunohistochemically in kidney tissue. The results showed that the SOD activity was lower in molybdenum group Ⅰthan that of the control group(P＜0.05), the SOD activity was lower significantly in molybdenum groupⅡthan that of the control group(P＜0.01);the MDA contends was higher in molybdenum groupⅡ than that of the control group in kidney tissue(P＜0.05); The expression of Smac of renal cortex was higher significantly in molybdenum group than that of the control group (P＜0.01). The results indicated that molybdenum may cause renal lipid peroxidation injury and promote expression of Smac protein in kidney tissue. |
| Keywords: | moybdenum kidney lipid peroxidation injury Smac protein mice |
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