| 北京鸭抑制素α-亚基免疫原的制备及活性研究 |
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| 引用本文： | 田锦.北京鸭抑制素α-亚基免疫原的制备及活性研究[J].中国畜牧兽医,2012,39(7):134-137. |
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| 作者姓名： | 田锦 |
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| 作者单位： | 北京农业职业学院,北京,102442 |
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| 基金项目： | 北京农业职业学院技术攻关与重点支持基金项目资助 |
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| 摘 要： | 将重组有北京鸭抑制素α-亚基基因的T载体和pET-28a表达载体分别用NdeⅠ和XhoⅠ双酶切后连接并转化E. coli BL21(DE3),经PCR和双酶切鉴定,筛选得到阳性重组表达载体,在IPTG诱导下融合表达了北京鸭抑制素α-亚基蛋白。试验结果表明,抑制素α-亚基基因在E. coli BL21中得到了有效表达,表达产物具有免疫活性,为后期探讨该免疫原提高北京鸭产蛋性能的研究奠定基础。
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| 关 键 词： | 北京鸭 抑制素α-亚基基因 免疫活性 |
| 收稿时间： | 2011-12-14 |
Study on Preparation and Immunocompetence of Inhibin Alpha-subunit in Beijing Duck |
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| TIAN Jin.Study on Preparation and Immunocompetence of Inhibin Alpha-subunit in Beijing Duck[J].China Animal Husbandry & Veterinary Medicine,2012,39(7):134-137. |
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| Authors: | TIAN Jin |
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| Institution: | Beijing Vocational College of Agriculture,Beijing 102442,China |
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| Abstract: | pMD18-T vector with the inhibin α-subunit gene from Beijing duck and pET-28a expression vector were cut by NdeⅠ and XhoⅠ.The products were connected and transformated into E.coli BL21(DE3).The positive recombinant expression vectors were identificated by PCR and double digestion experiment.The inhibin α-subunit protein was induced by the IPTG.Tests in mice showed that inhibin α-subunit expressed effectively in E.coli BL21 had immunological competence.The results are helpful for the later research about improving the laying performance of Beijing duck. |
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| Keywords: | Beijing duck inhibin α-subunit gene immunocompetence |
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