| 靶向env及LTR基因的siRNA抑制ALV-J 复制的研究 |
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| 引用本文： | 李娇,冯少珍,黄武,吴晓婵,李育豪,曹伟胜,廖明.靶向env及LTR基因的siRNA抑制ALV-J 复制的研究[J].中国畜牧兽医,2012,39(8):16-19. |
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| 作者姓名： | 李娇 冯少珍 黄武 吴晓婵 李育豪 曹伟胜 廖明 |
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| 作者单位： | 华南农业大学兽医学院,农业部兽用疫苗创制重点实验室,广东广州 510642 |
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| 基金项目： | NSFC-广东联合基金，国家肉鸡产业技术体系项目，广东省自然科学基金项目 |
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| 摘 要： | 试验针对J亚群禽白血病病毒的env及LTR基因,根据它们的保守序列,分别设计合成了4~5对siRNA,并将其克隆至pSilencer 4.1,构建siRNA重组表达质粒。将该质粒转染DF-1细胞6 h后以103 TCID50接种ALV-J,利用Real-time RT-PCR在mRNA表达水平检测各重组质粒对病毒复制的影响。结果表明,与阴性对照相比,pSi4.1-env各重组质粒对env基因的抑制效果达到18.15%~63.43%,pSi4.1-LTR各重组质粒的抑制效果则达到19.37%~45.41%。
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| 关 键 词： | J亚群禽白血病病毒 env基因 LTR基因 siRNA |
| 收稿时间： | 2012-02-07 |
siRNAs Targeted to env and LTR Gene for Inhibition of Avian Leukosis Virus Subgroup J Replication |
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| LI Jiao
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FENG Shao-zhen
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HUANG Wu
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WU Xiao-chan
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LI Yu-hao
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CAO Wei-sheng
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LIAO Ming.siRNAs Targeted to env and LTR Gene for Inhibition of Avian Leukosis Virus Subgroup J Replication[J].China Animal Husbandry & Veterinary Medicine,2012,39(8):16-19. |
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| Authors: | LI Jiao FENG Shao-zhen HUANG Wu WU Xiao-chan LI Yu-hao CAO Wei-sheng LIAO Ming |
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| Institution: | MOA Key Laboratory for Animal Vaccine Development,College of Veterinary Medicine, South China Agricultural University,Guangzhou 510642,China |
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| Abstract: | According to the env and LTR gene of avian leukosis virus subgroup J, 4 and 5 short hairpin RNAs (shRNAs) targeting their conserved sequences were designed systhessised and cloned into pSilencer 4.1 vector respectively. The recombinant plasmids were transfected into DF-1 cell and infected with 103 TCID50 ALV-J at 6 hours post-transfection, the efficiency of RNA interfering was assayed by Real-time RT-PCR .The results revealed that the recombinant plasmids of pSi4.1-env inhibited virus growth by 18.15% to 63.43% and pSi4.1-LTR by 19.37% to 45.41% compared to negative control. |
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| Keywords: | avian leukosis virus subgroup J virus env gene LTR gene siRNA |
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