| 猪伪狂犬病病毒SL株TK基因的克隆与生物信息学分析 |
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| 引用本文： | 张士强,易悦,徐志文,朱玲.猪伪狂犬病病毒SL株TK基因的克隆与生物信息学分析[J].中国畜牧兽医,2012,39(6):26-30. |
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| 作者姓名： | 张士强 易悦 徐志文 朱玲 |
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| 作者单位： | 四川农业大学动物医学院,四川雅安 625014 |
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| 基金项目： | 科技部国家科技支撑计划，教育部长江学者和创新团队发展计划项目 |
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| 摘 要： | 本试验对伪狂犬病病毒SL株(四川分离株)TK基因进行克隆,并对TK基因的同源性、遗传进化树、密码子偏嗜性、氨基酸结构及亲疏水性、跨膜区、信号肽进行了分析。结果显示,本研究成功克隆了PRV SL株TK全基因,其编码区长963 bp,编码320个氨基酸残基,与其他PRV分离株核苷酸序列同源性超过99.3%,编码的多肽链中疏水区域大于亲水区域,不含跨膜区及信号肽,不存在于病毒囊膜。
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| 关 键 词： | 伪狂犬病病毒 TK基因 生物信息学分析 |
Bioinformatics Analysis of TK Gene of Pseudorabies Virus SL Strain from Pigs |
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| ZHANG Shi-qiang
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YI Yue
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XU Zhi-wen
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ZHU Ling.Bioinformatics Analysis of TK Gene of Pseudorabies Virus SL Strain from Pigs[J].China Animal Husbandry & Veterinary Medicine,2012,39(6):26-30. |
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| Authors: | ZHANG Shi-qiang YI Yue XU Zhi-wen ZHU Ling |
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| Institution: | College of Veterinary Medicine,Sichuan Agricultural University,Ya’an 625014,China |
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| Abstract: | TK gene of pseudorabies virus SL strain was cloned. Based on the sequence, the homology, phylogeny tree, codon bias, amino acid structure, hydrophilic and hydrophobicity, transmembrane region and signal peptide were analyzed. The results showed that TK gene was successfully cloned and it consisted 963 nucleotides encoding a protein of 320 amino acids. The TK gene shared over 99.3% homology with that of the other PRV strains. PRV TK was predicted to not have the transmembrane region and signal peptide, and not be inside of virus envelope. |
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| Keywords: | pseudorabies virus TK gene bioinformatics analysis |
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