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| 2011年黄三角地区鸭源大肠杆菌的分离鉴定 | |
| 引用本文： | 李峰,林初文,张松林,苗立中,沈志强.2011年黄三角地区鸭源大肠杆菌的分离鉴定[J].中国畜牧兽医,2012,39(8):202-205. |
| 作者姓名： | 李峰 林初文 张松林 苗立中 沈志强 |
| 作者单位： | 1. 山东省滨州畜牧兽医研究院,山东滨州 256600; 2. 山东省兽医生物技术重点实验室,山东滨州 256600 |
| 摘 要： | 本试验从黄河三角洲区域2011年规模化肉鸭商品鸭场送检的病料中,通过细菌分离、培养特性观察、生化试验和致病性试验,分离到54株鸭源高致病性大肠杆菌。通过药敏试验,发现分离菌对多种抗生素存在多重耐药,其中对链霉素、氨苄西林和克林霉素3种药物的耐药性达到90%以上。敏感性较高的药物有阿米卡星、头孢噻肟、头孢曲松等药物。对分离菌进行了O抗原血清型分型,初步鉴定有12种血清型存在,其中O78、O35、O2、O18分别有12株、8株、6株和5株,为该区域优势血清型。本试验为了解和掌握该区域鸭源大肠杆菌病的流行情况,进一步研究和防控该病提供了参考依据。 |
| 关 键 词： | 黄三角 鸭源 大肠杆菌 分离 鉴定 |
| 收稿时间： | 2012-05-23 |
Isolation and Identification of the Yellow River Delta Regional Scale Meat Duck Intestines Goods in 2011 |
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| LI Feng , LIN Chu-wen , ZHANG Song-lin , MIAO Li-zhong , SHEN Zhi-qiang.Isolation and Identification of the Yellow River Delta Regional Scale Meat Duck Intestines Goods in 2011[J].China Animal Husbandry & Veterinary Medicine,2012,39(8):202-205. | |
| Authors: | LI Feng LIN Chu-wen ZHANG Song-lin MIAO Li-zhong SHEN Zhi-qiang |
| Institution: | 1. Shandong Binzhou Animal Science ＆Veterinary Medicine Academy, Binzhou 256600, China; 2. Shandong Key Laboratory of Veterinary Biotechnology, Binzhou 256600, China |
| Abstract: | This test separate to 54 plants duck highly pathogenic Escherichia coli source by the bacteria isolation, culture characteristic observation and biochemical test and pathogenic test, epidemic materials come from the Yellow River delta regional scale meat duck intestines goods in 2011. The drug sensitivity test found that separation strains of several antibiotics exist multiple resistance, wherein, three drug resistance of Phytomycin, Ampicillin and Clindamycin reached more than 90%. Higher sensitivity of drugs have Amikacin, Cefotaxime, Ceftriaxone and so on. This test identify preliminarily 12 kinds of serum type exist through O serum antigen type classification, including the region superiority serotype of O78, O35, O2, O18 were 12 strains, 8 strains, 6 strains and 5 strains. This test provide the reference for understanding and grasp the duck source area the epidemic of Escherichia coli disease and further research and prevention and control the disease. |
| Keywords: | the Yellow River delta regional duck Escherichia coli isolation identification |
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