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| 应用逆转录套式PCR检测猪流行性腹泻病毒 | |
| 引用本文： | 王金良,谢金文,唐娜,祖立闯,李娇,汪洋,沈志强.应用逆转录套式PCR检测猪流行性腹泻病毒[J].中国畜牧兽医,2012,39(9):86-88. |
| 作者姓名： | 王金良 谢金文 唐娜 祖立闯 李娇 汪洋 沈志强 |
| 作者单位： | 1. 山东省滨州畜牧兽医研究院,山东滨州 256600; 2. 山东绿都生物科技有限公司,山东滨州 256600 |
| 基金项目： | 山东省自然科学基金资助项目 |
| 摘 要： | 根据已发表的猪流行性腹泻病毒(PEDV)N基因核苷酸序列,设计合成2对引物,其中外引物扩增片段长度为297 bp,内引物扩增片段长度为212 bp,在优化RT-PCR反应条件的基础上,建立了检测PEDV的套式RT-PCR检测方法,该方法较普通RT-PCR更加灵敏、可靠,能有效降低假阳性和污染,可用于PEDV的快速诊断和流行病学调查。 |
| 关 键 词： | 猪流行性腹泻病毒 N基因 套式RT-PCR 检测 |
| 收稿时间： | 2012-03-21 |
Detection of Porcine Epidemic Diarrhea Virus by RT-nested PCR |
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| WANG Jin-liang , XIE Jin-wen , TANG Na , ZU Li-chuang , LI Jiao , WANG Yang , SHEN Zhi-qiang.Detection of Porcine Epidemic Diarrhea Virus by RT-nested PCR[J].China Animal Husbandry & Veterinary Medicine,2012,39(9):86-88. | |
| Authors: | WANG Jin-liang XIE Jin-wen TANG Na ZU Li-chuang LI Jiao WANG Yang SHEN Zhi-qiang |
| Institution: | 1. Shandong Binzhou Academy of Animal Science and Veterinary Medicine, Binzhou 256600, China; 2. Shandong Lvdu Biological Technology Co., Ltd., Binzhou 256600, China |
| Abstract: | Based on the published nucleotide sequence of porcine epidemic diarrhea virus N gene,two pairs of primers were designed and synthesized,which outer primers amplified fragment length was 297 bp,and inner primers amplified fragment length was 212 bp.RT-nested PCR methods had been established through optimization of the RT-PCR reaction conditions,this method was more sensitive and reliable than ordinary RT-PCR,and could effectively reduce the false positive and pollution.It could be used for rapid diagnosis and epidemiological investigation of the PEDV. |
| Keywords: | PEDV N gene RT-nested PCR detection |
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