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| 通直型巨龙竹不同地理种源遗传分化的ISSR分析 | |
| 引用本文： | 杨汉奇,阮桢媛,田波,杨宇明,孙茂盛.通直型巨龙竹不同地理种源遗传分化的ISSR分析[J].浙江林学院学报,2010,27(1):81-86. |
| 作者姓名： | 杨汉奇 阮桢媛 田波 杨宇明 孙茂盛 |
| 作者单位： | 1. 中国林业科学研究院,资源昆虫研究所,云南,昆明,650224 2. 西南林学院,资源学院,云南,昆明,650224 3. 中国科学院,西双版纳热带植物园昆明分部,云南,昆明,650223 |
| 基金项目： | "十一五"国家科技支撑计划项目，国际竹藤网络中心基本科研业务费专项资金项目，云南省科学技术厅联合支持国家科技项目 |
| 摘 要： | 通直型巨龙竹Dendrocalamus sinicus是云南重要的经济竹种资源之一。为了保护和开发通直型巨龙竹种质资源．应用简单序列重复区间扩增（ISSR）标记对通直型巨龙竹核心分布区不同地理种源的遗传变异进行了研究。从80个引物中筛选出7个用于正式扩增，在所研究的4个居群共54个个体中检测到54个多态位点。在居群水平上。多态位点百分率为9．59％，Nei’s基因多样性指数（H）和Shannon信息指数（I）分别为0．0363和0．0536：在物种水平上，多态位点百分率为73．97％，H和I分别为0．2600和0．3921。居群间的遗传分化系数（Ga）达0．8634，显示不同居群间遗传分化很大。造成通直型巨龙竹不同地理种源遗传分化的主要原因可能是生境片断化和自然条件下花而不实造成的种子传播困难．图4表4参21 |
| 关 键 词： | 林木育种学 巨龙竹 遗传分化 简单序列重复区间扩增 |
Genetic differentiation of four straight-culmed provenances of Dendrocalamus sinicus based on ISSRs |
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| YANG Han-qi,RUAN Zhen-yuan,TIAN Bo,YANG Yu-ming,SUN Mao-sheng.Genetic differentiation of four straight-culmed provenances of Dendrocalamus sinicus based on ISSRs[J].Journal of Zhejiang Forestry College,2010,27(1):81-86. | |
| Authors: | YANG Han-qi RUAN Zhen-yuan TIAN Bo YANG Yu-ming SUN Mao-sheng |
| Institution: | YANG Han-qi1,RUAN Zhen-yuan2,TIAN Bo3,YANG Yu-ming2,SUN Mao-sheng2(1.Research Institute of Resource Insects,The Chinese Academy of Forestry,Kunming 650224,Yunnan,China,2.School of Resources,Southwest Forestry College,3.Xishuangbanna Tropical Botanical Garden,Chinese Academy of Sciences,Kunming 650223,China) |
| Abstract: | The straight-culmed type of Dendrocalamus sinicus is one of the most important economic bam-boo resources in Yunnan Province, China. To protect and develop native germplasm resources of the straight-culmed D. Sinicus in Yunnan, the genetic diversity of four straight-culmed provenances of D. Sini-cus was analyzed based on inter-simple sequence repeat(ISSR) markers for percentage of polymorphic bands (PPB), Nei's gent diversity(H), Shannon's information index(I) and Nei's coefficient of genetic differ-entiation(G_(st)). Results showed seven informative and reliable primers with 54 polymorphic loci that were cho-sen from 80 pre-screened primers. At the population level, PPB was 9.59%, H was 0.036 3, and I was 0.053 6; whereas at the species level, PPB was 73.97%, H was 0.260 0 and I was 0.3921. Also, G, was 0.863 4. G_(st) indicated a high degree of genetic differentiation among straight-culmed D. Sinicus provenances most likely due to habitat fragmentation and flowering without seed. |
| Keywords: | forest tree breeding Dendrocalamus sinicus genetic differentiation inter-simple sequence repeat (ISSR) |
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