A comparison of genetic relationship measures in strawberry (Fragaria × ananassa Duch.) based on AFLPs, RAPDs, and pedigree data |
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| Authors: | Chemda Degani Lisa J Rowland James A Saunders Stan C Hokanson Elizabeth L Ogden Avi Golan-Goldhirsh Gene J Galletta |
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| Institution: | (1) Institute of Horticulture, Agricultural Research Organization, The Volcani Center, Bet-Dagan, 50250, Israel;(2) Fruit Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, Beltsville, MD, 20705, U.S.A.;(3) Climate Stress Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, Beltsville, MD, 20705, U.S.A.;(4) Desert Plant Biotechnology Laboratory, The Jacob Blaustein Institute for Desert Research, Ben-Gurion University of the Negev, Sede Boker Campus, 84990, Israel |
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| Abstract: | Nineteen of the major strawberry (Fragaria × ananassa Duch.) cultivars grown in the UnitedStates and Canada were examined for AFLP markerpolymorphisms. For the AFLP reactions, the EcoRI-ACC primer was used in combination with fourMseI primers (MseI-CAC, MseI-CAG,MseI-CAT, or MseI-CTT). Each set ofprimers produced 46–66 scorable fragments ranging insize between 50 and 500 bp. The polymorphic fragmentsproduced from each set of primers were more thansufficient to distinguish among all the cultivars,demonstrating the usefulness of AFLP markers forcultivar identification. Similarity coefficients werecalculated based on data from 228 AFLP markers anddata from 15 previously characterized RAPD markers. The RAPD markers had been specifically selected forfingerprinting purposes because they succesfullydistinguish 41 strawberry cultivars, including the 19cultivars analyzed in this study. Separatedendrograms were constructed based on analysis of theAFLP and RAPD marker data using a neighbor-joiningalgorithm. The dendrograms were compared and found tobe very different. Correlations between similaritycoefficients calculated from AFLP marker data,similarity coefficients calculated from RAPD markerdata, and coefficients of coancestry calculated frompedigree information were evaluated. Interestingly,a better correlation with the coefficients ofcoancestry was observed with the RAPD marker data thanwith the AFLP marker data. |
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| Keywords: | AFLP markers DNA fingerprinting Fragaria × ananassa genetic diversity genetic relationships strawberry |
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