| 红苋R104种子谷蛋白亚基组成及二硫键连接蛋白研究 |
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| 引用本文： | 宋宏新,汪沛洪.红苋R104种子谷蛋白亚基组成及二硫键连接蛋白研究[J].西北农林科技大学学报(社会科学版),1996,24(3):5-9. |
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| 作者姓名： | 宋宏新 汪沛洪 |
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| 作者单位： | 西北农业大学生化研究室 |
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| 摘 要： | 应用还原和非还原单向ＳＤＳ－ＰＡＧＥ及二者结合的双向电泳技术，研究了红苋Ｒ１０４种子谷蛋白的亚基组成。谷蛋白由多种亚基组成，高分子量二硫键连接蛋白占其多数，经还原裂解成Ａ（５４ＫＤ）、Ｂ（４０ＫＤ，３９ＫＤ）、Ｃ（３３ＫＤ，３１ＫＤ）、Ｄ（２２ＫＤ，２０ＫＤ，１８ＫＤ，）和Ｅ（１５ＫＤ）５组主要单肽链单元，提出了二硫键连接蛋白的组成模式，并发现低分子量谷蛋白亚基存在肽内二硫键
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| 关 键 词： | 籽粒苋 谷蛋白 双向电泳 二硫键连接蛋白 |
Disulphide-linked Proteins and Subunit Composition of the Glutelin of Amaranth credentus R104 |
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| Song Hongxin.Disulphide-linked Proteins and Subunit Composition of the Glutelin of Amaranth credentus R104[J].Journal of Northwest Sci-Tech Univ of Agr and,1996,24(3):5-9. |
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| Authors: | Song Hongxin |
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| Institution: | (Northwestern Agricultural University, Yangling, Shaanxi,712100) |
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| Abstract: | Under non reducing or reducing conditions,the onedimensional or two dimensional methods were employed in this research on the glutelin of A. credentus R104 seed.The results showed that the glutelin consisted of many subunits.The majority of the glutelin was HMW disulphide linked proteins,which could be dissociated into five groups by reducing: A(54 KD), B(40 KD, 39 KD),C(33 KD,31 KD),D(22 KD,20 KD,18 KD),and E(15 KD).So the HMW protein composition model was put forward accordingly. |
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| Keywords: | grain amaranth seed protein glutelin electrophoresis disulphide linked proteins |
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