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| 山核桃愈伤组织诱导的初步研究 | |
| 引用本文： | 朱玉球,廖望仪,黄坚钦,孙晓萍.山核桃愈伤组织诱导的初步研究[J].浙江林学院学报,2001,18(2):115-118. |
| 作者姓名： | 朱玉球 廖望仪 黄坚钦 孙晓萍 |
| 作者单位： | 1. 浙江林学院资源与环境系, 2. 浙江森禾种业有限公司金华分公司, 3. 浙江省杭州市园林文物局苗圃, |
| 基金项目： | 浙江省自然科学基金资助项目(300011) |
| 摘 要： | 采用正交设计研究了不同植物激素配比、蔗糖质量浓度、外植体取材部位以及单宁含量等因素对山核桃组织培养中愈伤组织形成的影响。结果表明：WPM＋BA8．0mg.L^-1 IB2.0mg.L^-1 GA1.0mg.L^-1＋蔗糖30mg.L^-1是最适培养基；顶芽和第4芽段为较适宜的山核桃愈伤组织诱导的取材部位；不同芽段愈伤组织的形成率与其单宁含量不相关，而与芽段愈伤组织形成的部位有关，且随着单宁含量的减少。愈伤组织形成的部位向切口上升。选取1年生实生苗茎尖和茎段，配比合理的植激素加以诱导，可提高山核桃嫁接成活率。表5参11。 |
| 关 键 词： | 山核桃 组织培养 愈伤组织 单宁 嫁接 诱导 |
| 文章编号： | 1000-5692(2001)02-0115-04 |
A preliminary study on induction of callus in Carya cathayensis |
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| ZHU Yu-qiu,LIAO Wang-yi,HUANG Jian-qin,SUN Xiao-ping.A preliminary study on induction of callus in Carya cathayensis[J].Journal of Zhejiang Forestry College,2001,18(2):115-118. | |
| Authors: | ZHU Yu-qiu LIAO Wang-yi HUANG Jian-qin SUN Xiao-ping |
| Abstract: | Effects of such factors as ratios of different plant hormones，mass concentrations of surose，the part from which explants were obtained and the content of tannins on the formation of callus in the tissue culture of Carya cathayensis were studied by means of an orthogonal design．It's showed that WPM＋BA 8.0 mg*L－1＋IBA 2.0 mg*L－1＋GA 1.0 mg*L－1＋sucrose 30 mg*L－1 was an optimal medium；the portion between apical buds and the 4th bud was suitable to induce callus in Carya cathayensis；and the formation rate of callus for loci of the buds was not correlated with the content of tannins，but in correlation with the loci where callus was formed．In addition，the smaller the content of tannins，the farther the locus where callus formed was from the cut．The key of raising survival rate of grafting was the selection of scions and the proportions of hormones to be used in grafting． |
| Keywords: | Carya cathayensis tissue culture callus tannin grafting |
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