| ISSR分子标记分析杏鲍菇菌株遗传差异研究 |
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| 引用本文： | 冯伟林,蔡为明,金群力,范丽军.ISSR分子标记分析杏鲍菇菌株遗传差异研究[J].中国食用菌,2009,28(1). |
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| 作者姓名： | 冯伟林 蔡为明 金群力 范丽军 |
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| 作者单位： | 1. 浙江省农业科学院冈艺所,浙江,杭州,310021;浙江大学农业与生物技术学院,浙江,杭州,310029
2. 浙江省农业科学院冈艺所,浙江,杭州,310021 |
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| 摘 要： | 试验应用ISSR技术分析了杏鲍菇生产性菌株的DNA序列多态性,从20个引物中筛选出11个ISSR引物对12个杏鲍菇生产性菌株基因组DNA进行PCR扩增。结果表明,这些杏鲍菇菌株遗传相似系数为0.68～1.00。采用平均分类法UPGMA分析表明,在遗传相似系数为0.83处可将这12个杏鲍菇生产性菌株分成3类。
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| 关 键 词： | 杏鲍菇 遗传差异 ISSR |
Analysis of Genetic Variation of Pleurotus eryngii Strains by ISSR Marker |
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| FENG Wei-lin,CAI Wei-ming,JIN Qun-li,FAN Li-jun.Analysis of Genetic Variation of Pleurotus eryngii Strains by ISSR Marker[J].Edible Fungi of China,2009,28(1). |
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| Authors: | FENG Wei-lin CAI Wei-ming JIN Qun-li FAN Li-jun |
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| Institution: | 1 (1.Institute of Horticulture Zhejiang Academy of Agricultural Sciences; Hangzhou Zhejiang 310021; 2.College of Agricultural and Biological Technology; Zhejiang University; Hangzhou Zhejiang 310029); |
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| Abstract: | Inter-simple sequence reqeats (ISSR) methods were used to analyze the DNA sequence polymorphism of cultivated Pleurotus erngii strains. 11 primers were chosed for ISSR among 20 primers. Genomic DNA of Pleurotus erngii were amplified by 11 primers using PCR. The results showed that the genetic similar coefficients (GSC) were 0.68~1.00. Cultivated strains were divided into 3 groups at GSC 0.83 based on the cluster analysis of UPGMA method. |
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| Keywords: | ISSR |
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