| 淡紫拟青霉NH-PL-03菌株在不同培养基上的生长特性 |
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| 引用本文： | 李芳,黄素芳,刘波.淡紫拟青霉NH-PL-03菌株在不同培养基上的生长特性[J].植物保护,2005,31(3):46-49. |
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| 作者姓名： | 李芳 黄素芳 刘波 |
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| 作者单位： | 1. 福建农林大学植物保护学院,福建,福州,350002
2. 福建农业科学院生物技术中心,福建,福州,350003 |
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| 基金项目： | 国家863计划项目(2002AA244031-2)，福建省计委项目闽计投资([2003]170号). |
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| 摘 要： | 观察淡紫拟青霉菌在不同培养基上的生长状况,研究淡紫拟青霉菌在不同培养基上的生长特性及对茎线虫的毒力。结果表明:不同培养基对淡紫拟青霉NH-PL-03菌株的生长特性有明显影响。在孟加拉红、PDA和察氏培养基上,菌丝量增长最为显著,培养8d,菌丝量分别达到0.800、.73 g/50 mL和0.70g/50 mL;培养过程产孢量处于动态增长趋势,而且不同培养基产孢量大不相同;在察氏和燕麦培养基上产孢量最大,培养8d,产孢量分别达到36.90×106/mL和35.51×106/mL;培养液滤液的毒力随淡紫拟青霉培养时间延长而升高,在察氏培养基培养毒力较高,对茎线虫的最高校正死亡率达到43.44%。
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| 关 键 词： | 有害生物生物防治 淡紫拟青霉 培养基 生长特性 毒力 |
Growing characteristics of Paecilomyces lilacinus (Thom.) Samson NH-PL-03 on different media |
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| LI Fang;HUANG Su-fang;LIU Bo.Growing characteristics of Paecilomyces lilacinus (Thom.) Samson NH-PL-03 on different media[J].Plant Protection,2005,31(3):46-49. |
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| Authors: | LI Fang;HUANG Su-fang;LIU Bo |
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| Institution: | 1. College of Plant Protection;Fujian University of Agriculture and Forestoy;Fuzhou 350002;China; 2.Biotechnology center of Fujian Academy of Agricultural Sciences;Fuzhou 350003;China |
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| Abstract: | The OD value, pH value, dry weight of mycelia, sporulation and toxic affect on nematode in the process of culture have been investigated to studied the growing characteristics on different media. Result showed that Paecilomyces lilacinus could grow normally on all tested media. Best mycelia growth occurs on the media of Martin & Johnson ,PDA,and Czapek, cultured 8d,the dry weight of mycelia could reach 0.80g/ 50ml,0.73g/ 50ml and 0.70g/ 50ml respectively; The maximun sporulation of 36.90×106/ml produced on Czapek media,the next was oat media(35.51×106/ml). The filtrate has significant toxic effects on Ditylenchus destructor Thorne, the revised mortality of treated juveniles increase along with culture continues, the maximun. revised mortality of D. destructor treated by filtrate from Czapek media was 43.44%. |
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| Keywords: | pest bio-control Paecilomyces lilacinus media culture characteristics toxic effect |
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