| 供体细胞的不同处理方法对牛核移植重构胚发育能力的影响 |
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| 引用本文： | 梁素丽,李向臣,白修云,王有柱,关伟军.供体细胞的不同处理方法对牛核移植重构胚发育能力的影响[J].中国畜牧兽医,2007,34(8):53-57. |
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| 作者姓名： | 梁素丽 李向臣 白修云 王有柱 关伟军 |
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| 作者单位： | 1.西北农林科技大学动物科技学院，杨凌 712100;2.中国农业科学院北京畜牧兽医研究所，北京 100094 |
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| 摘 要： | 为了研究供体细胞不同的处理方法对核移植重构胚的作用，比较了用于保种的冻存和新鲜的成纤维细胞做供体细胞、供体细胞不同的离心转数、供体细胞不同的血清饥饿时间及用本实验室冻存的成纤维细胞，解冻复苏后，不同传代次数对重构胚的影响。结果表明分别用冷冻保存的和新鲜的成纤维细胞作为核供体，所得重构胚卵裂率、囊胚率无显著差（P>0.05）；供体细胞离心800 r/min时所得重构胚效果较好，离心1500 r/min所得重构胚的卵裂率、囊胚率与其他3组相比较低；血清饥饿3～5 d组所得重构胚比其他3组好；用解冻复苏后再传2、5代的细胞进行核移植，所得重构胚的卵裂率、囊胚率无明显差异(P>0.05)，显著高于传8代的细胞所得重构胚。说明供体细胞的不同处理方法对核移植重构胚发育有很重要的影响。
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| 关 键 词： | 供体细胞 牛 核移植 重构胚 |
| 文章编号： | 1671-7236（2007）08-0053-05 |
| 修稿时间： | 2007-03-08 |
The Influence of Donor Cell Treatments on the Development of Nuclear Transfer Embryos of Bovine |
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| LIANG Su-li,LI Xiang-chen,BAI Xiu-yun,WANG You-zhu,GUAN Wei-jun.The Influence of Donor Cell Treatments on the Development of Nuclear Transfer Embryos of Bovine[J].China Animal Husbandry & Veterinary Medicine,2007,34(8):53-57. |
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| Authors: | LIANG Su-li LI Xiang-chen BAI Xiu-yun WANG You-zhu GUAN Wei-jun |
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| Institution: | 1.Northwest Sci Tech University of Agriculture and Forestry, Yangling 712100, China;2.lnstitute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China |
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| Abstract: | In order to study the influence of Donor Cells Treatments on the Development of nuclear transfer embryos of Bovine. The influence of cryopreservative and fresh fibroblast cells、different Centrifugal speed of donor cell、different serum starvation and passage time of cryopreservative fibroblast cells after thawing were studied. The cleavage and blastocyst rate of nuclear transfer embryos derived from cryopreservative and fresh fibroblast cells were not significantly different（P>0.05）; The cleavage and blastocyst rate of nuclear transfer embryos derived from donor cell treated with centrifugal speed 1500 r/min was significantly lower than other groups;The blastocysts that obtained from 3～5 d serum starvation is highter than other group. The cleavage and blastocyst rate of nuclear transfer embryos from passage 8 times after thawing is lowest compared with passage 2 and 5 times. Different treatment of donor cell have impotent effects for nuclear transfer embryos. |
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| Keywords: | donor cell bovine nuclear transfer embryo of NT |
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