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Molecular Cloning and Characterization of the Actin-depolymerizing Factor Gene in Gossypium barbadense
Authors:MA Zhi-ying  CHI Ji-na  WANG Xing fen  ZHOU Hong-mei  ZHANG Gui-yin
Abstract:Sea Island cotton (Gossypium barbadense L.) has been highly valued in Verticillium wilt resistance and many fiber qualities including fiber length,strength,and fineness.To identify whether it had some special genes in fiber development in comparison with the upland cotton (G.hirsutum L.),an actin-depolymerizing factor (ADF) gene was cloned and characterized in this research.A 420 bp open reading frame of the cloned gene,termed GbADF1,encoded a protein of 139 amino acids,which included39.57% nonpolar amino acids,17.27% acidic amino acids,15.83% basic amino acids,and 31.92% hydrophobic amino aids.
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