| 高原作物青稞根及根际内生细菌群落结构的高通量分析 |
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| 引用本文： | 刘振东,王波,薛蓓,王强锋,侯磊.高原作物青稞根及根际内生细菌群落结构的高通量分析[J].作物杂志,2016,32(6):49-13. |
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| 作者姓名： | 刘振东 王波 薛蓓 王强锋 侯磊 |
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| 作者单位： | 1 西藏农牧学院,860000,西藏林芝2 西藏自治区农牧科学院农业研究所,850032,西藏拉萨3 四川省农业科学院生物技术核技术研究所,610061,四川成都 |
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| 基金项目： | 西藏自治区自然科学基金(2015212-14-23);西藏自治区青年教师创新计划项目(QCZ2016-45);国家现代农业(大麦青稞)产业技术体系加工试验站(CARS-05) |
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| 摘 要： | 利用高通量测序技术分析了西藏青稞主栽品种藏青2000根及根际内生细菌的16S V4变异区序列,同时应用UparseRON、KA等软件整理和统计样品序列数和操作分类单元（OTU）数,分析物种丰度及Alpaha多样性等指标。获得的用于分析青稞根部内生细菌的有效序列和OTUs为55 192/3 613,用于分析青稞根际土壤内生细菌的有效序列和OTUs为62 181/4 399;根部及根际土壤Chao1指数分别为3 443.4290和4 632.5820,shannon多样性指数分别为8.542和9.967。在门水平对代表性OTU进行注释发现,青稞根及根际土壤内生细菌主要是变形菌门（Proteobacteria）、拟杆菌门（Bacteroidetes）、放线菌门（Actinobacteria）、酸杆菌门（Acidobacteria）、厚壁菌门（Firmicutes）。在属水平上主要是假单胞菌属（Pseudomonas）、链霉菌属（Streptomyces）、土地杆菌属（Pedobacter）、黄杆菌属（Flavobacterium）、红游动菌属（Rhodoplanes）。结果显示,青稞根及根际内生细菌具有丰富的多样性,且二者的优势菌属一致。
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| 关 键 词： | 青稞 根 根际 内生细菌 种类 丰度 |
| 收稿时间： | 2016-08-27 |
High Throughput Analysis of the Endophytic Bacterial Community Structure in Roots and Rhizosphere of Highland Barley of Tibetan Plateau |
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| Zhendong Liu,Bo Wang,Bei Xue,Qiangfeng Wang,Lei Hou.High Throughput Analysis of the Endophytic Bacterial Community Structure in Roots and Rhizosphere of Highland Barley of Tibetan Plateau[J].Crops,2016,32(6):49-13. |
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| Authors: | Zhendong Liu Bo Wang Bei Xue Qiangfeng Wang Lei Hou |
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| Institution: | 1 Tibet Agricultural and Animal Husbandry College,Nyingchi 860000,Tibet,China2 Agricultural Institute,Tibet Academy of Agricultural and Animal Husbandry Sciences,Lhasa 850032,Tibet,China3 Institute of Biological & Nuclear Technology,Sichuan Academy of Agricultural Sciences,Chengdu 610061,Sichuan,China; |
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| Abstract: | High-throughput sequencing technology has been applied to the determination of 16S V4 variation sequences of the endophytic bacteria in roots and rhizosphere of the main cultivar of highland barley (Zangqing 2000) in Tibetan Plateau. The number of sample sequences and operational taxonomic units (OTUs) were sorted and calculated and the species richness and alpha diversity index were analyzed by Uparse and KRONA. The results showed that the useful sequences and OTUs of the endophytic bacteria in roots were 55 192/3 613, while the useful sequences and OTUs of the endophytic bacteria in rhizosphere were 62 181/4 399. The Chao1 indices of the endophytic bacteria in roots and rhizosphere were 3 443.4290 and 4 632.5820, respectively, and the Shannon diversity indices were 8.542 and 9.967, respectively. The endophytic bacteria in roots and rhizosphere of highland barley were mainly included in Pseudomonas, Streptomyces, Pedobacter, Flavobacterium and Rhodoplanes. These results indicate that the endophytic bacteria in roots and rhizosphere of highland barley ZQ2000 have rich diversity and the dominant genera of the bacteria in both roots and rhizosphere. |
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| Keywords: | Highland barley Root Rhizosphere Endophytic bacteria Species Richness |
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