| 过硫酸氢钾干预下对虾养殖系统中水质指标和菌群结构变化分析 |
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| 引用本文： | 蔡欣欣,于永翔,王印庚,张正,廖梅杰,李彬,刘潇,朱洪洋,荣小军.过硫酸氢钾干预下对虾养殖系统中水质指标和菌群结构变化分析[J].上海海洋大学学报,2022,31(2):452-461. |
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| 作者姓名： | 蔡欣欣 于永翔 王印庚 张正 廖梅杰 李彬 刘潇 朱洪洋 荣小军 |
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| 作者单位： | 上海海洋大学,中国水产科学研究院黄海水产研究所,中国水产科学研究院黄海水产研究所,中国水产科学研究院黄海水产研究所,中国水产科学研究院黄海水产研究所,中国水产科学研究院黄海水产研究所,中国水产科学研究院黄海水产研究所,中国水产科学研究院黄海水产研究所,中国水产科学研究院黄海水产研究所 |
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| 基金项目： | 国家重点研发计划课题（2019YFD0900102）；山东省泰山产业领军人才项目（LJNY201802）；苏北科技专项（SZ-LYG202028） |
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| 摘 要： | 摘要：该文通过水质理化因子监测、虾体和水体中可培养细菌和弧菌检测及高通量测序方法，系统解析过硫酸氢钾（PMS）干预下对虾养殖池塘水体环境指标和菌群结构的变化情况。结果表明：施用0.2 g/L PMS对对虾养殖水体中低含量氨氮也具有一定改善作用，泼洒PMS 24h和72h后的水温、溶解氧、pH、盐度、亚硝酸盐无显著差异。PMS泼洒后对虾肝胰腺内可培养细菌和弧菌数量分别由3.13×106 CFU/g和1.98×106 CFU/g降低至4.30×105 CFU/g和1.09×105 CFU/g，弧菌占比由63.36%降低至25.35%；水体中可培养细菌和弧菌数量分别由2.70×104 CFU/mL和6.00×103 CFU/m降低至8.50×103 CFU/mL和1.20×103 CFU/mL，弧菌占比由22.22%降低至14.11%，PMS可显著降低虾体和水体中可培养细菌数量及弧菌占比。对水体菌群结构进行高通量测序分析表明，放线菌门、拟杆菌门、蓝藻门和变形菌门为主要优势菌门，泼洒PMS前后（PB1/PA1），腈基降解菌科、PeM15、DS001、Llumatobacteraceae、微杆菌科、红细菌科相对丰度显著升高（P<0.05），巴纽尔斯菌科、腐螺菌科、Stappiaceae相对丰度显著降低（P<0.05），对比泼洒PMS前后3天的变化趋势表明，泼洒PMS后池塘优势菌相对丰度发生显著变化且菌群结构PCoA指数偏离度较低。相关研究结果为评判PMS在水产养殖中的防控作用及其科学使用提供数据支撑。
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| 关 键 词： | 凡纳滨对虾 过硫酸氢钾 水质理化因子 可培养细菌 弧菌 菌群结构 |
| 收稿时间： | 2021/2/2 0:00:00 |
| 修稿时间： | 2021/4/11 0:00:00 |
Effects of potassium monopersulfate on water environment index and microbial community structure of Litopenaeus vannamei in pond culture system |
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| CAI Xinxin,YU Yongxiang,WANG Yingeng,ZHANG Zheng,LIAO Meijie,LI Bin,LIU Xiao,ZHU Hongyang,RONG Xiaojun.Effects of potassium monopersulfate on water environment index and microbial community structure of Litopenaeus vannamei in pond culture system[J].Journal of Shanghai Ocean University,2022,31(2):452-461. |
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| Authors: | CAI Xinxin YU Yongxiang WANG Yingeng ZHANG Zheng LIAO Meijie LI Bin LIU Xiao ZHU Hongyang RONG Xiaojun |
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| Institution: | Shanghai Ocean University,Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences,Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences,Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences,Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences,Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences,Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences,Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences,Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences |
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| Abstract: | The effects of potassium monopersulfate (PMS) on water environment index and bacterial community were analyzed by water quality physical and chemical factors monitor, culturable bacteria detection in shrimp hepatopancreas and water, and bacterial community analysis. The results showed that:compared with the control, the application of 0.2 g/L PMS could also improve the low content of ammonia nitrogen (NH4-N) in shrimp culture water. There was no significant difference in water temperature, dissolved oxygen, pH, salinity and nitrite (NO2-N) after spraying PMS for 24 h and 72 h.The number of culturable bacteria and Vibrio spp. in hepatopancreas decreased from 3.13×106 CFU/g and 1.98×106 CFU/g to 4.30×105 CFU/g and 1.09×105 CFU/g respectively, and the proportion of Vibrio spp. decreased from 63.36% to 25.35%. The number of culturable bacteria and Vibrio spp. in water decreased from 2.70×104 CFU/mL and 6.00×103 CFU/mL to 8.50×103 CFU/mL and 1.20×103 CFU/mL respectively, and the proportion of Vibrio spp. decreased from 22.22% to 14.11%. PMS could significantly reduce the number of culturable bacterias and the proportion of Vibrios spp. in hepatopancreas and pond water. Bacterial community analysis of the water showed that Actinobacteria, Bacteroidota, Cyanobacteria and Proteobacteria were the main dominant phyla. The relative abundance of Nitriliruptoraceae,PeM15,DS001,Llumatobacteraceae,Microbacteriaceae and Rhodobacteraceae was significantly increased after PMS sprayed (P<0.05) (PB1/PA1), and the relative abundances of Barnulmaceae,Saprospiraceae and Stappiaceae were significantly decreased (P<0.05). Compared with the variation trends of 3 days before and after PMS interference, the difference of bacterial community structure between PMS free groups was gradually increased, and reflected that the PMS has better stability in maintaining the bacterial structure of the aquaculture pond. The results may provide data support for the prevention roles and scientific application of PMS in aquaculture. |
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| Keywords: | Litopenaeus vannamei Potassium monopersulfate Water environment index Culturable bacteria Vibrios Bacterial community |
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