| 长白山区土壤中高毒力苏云金杆菌的分离及生物测定 |
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| 引用本文： | 李真,金大勇.长白山区土壤中高毒力苏云金杆菌的分离及生物测定[J].农业科学与技术,2017,18(3). |
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| 作者姓名： | 李真 金大勇 |
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| 作者单位： | 延边大学农学院,吉林延吉,133002 |
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| 基金项目： | 延边大学自然基金项目(2011-34).Supported by Natural Science Foundation of Yanbian University |
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| 摘 要： | 目的]分离筛选出对鳞翅目害虫高毒力的新苏云金杆菌.方法]通过温度筛选法从长白山区土壤中分离出苏云金杆菌,再通过生物测定和毒力测定筛选出高毒力苏云金杆菌.结果]从150份土壤样品中分离出18株苏云金杆菌,其分离率为12.0％,其中山地分离率为8.5％,农田分离率为16.2％.活性测定结果,18株菌株中对小菜蛾、斜纹夜蛾及甜菜夜蛾致死率超过90％的菌株分别有17株、5株和4株,其中YN1-1菌株对3种害虫都表现出高活性.毒力测定结果,YN1-1菌株对甜菜夜蛾和斜纹夜蛾的速效性最好;对甜菜夜蛾高毒力菌株依次为YN6-2＞YN1-1 ＞YN4-2＞YN2-6;对斜纹夜蛾高毒力菌株依次为YN4-4＞YN1-1 ＞YN6-1 ＞YN4-1＞YN2-1.结论]经活性测定和毒力测定,最终筛选出广谱性、速效性及高毒力的YN1-1为目标菌株.
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| 关 键 词： | 苏云金杆菌 生物测定 活性测定 毒力测定 |
Isolation and Biological Determination of Bacillus thuringiensis with High Toxicity in Soil of Changbai Mountain Area |
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| Zhen LI,Dayong JIN.Isolation and Biological Determination of Bacillus thuringiensis with High Toxicity in Soil of Changbai Mountain Area[J].Agricultural Science ＆ Technology,2017,18(3). |
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| Authors: | Zhen LI Dayong JIN |
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| Abstract: | Objective] This study was conducted to isolate and screen new Bacillus thuringiensis (Bt) strains against Lepidoptera insecticides.Method] Bt strains were isolated from soil of Changbai Mountain area by temperature screening method,and highly-toxic Bt stains were then selected by biological determination and toxicity determination.Result] From 150 soil samples,18 Bt isolates were isolated,with an average isolation rate of 12.0％.Specifically,the isolation rate from mountain field was 8.5 ％,and the isolation rate from farmland was 16.2％.The results of activity determination showed that there were17,5 and 4 strains showing lethality rate over 90％ against Plutella xylosrella,Spodoptera litura and Spodptera exigua,respectively,and among them,strain YN1-1 exhibited high activity against all the 3 kinds of insects.The results of toxicity determination showed that strain YN1-1 had the best fast-acting property against S.litura and S.exigua;strains with high toxicity against S.exigua were YN6-2＞YN1-1＞YN4-2＞YN2-6 sequentially;and toxicity of strains against S.litura was in order of YN4-4＞YN1-1 ＞YN6-1 ＞YN4-1 ＞YN2-1.Conclusion]According to activity determination and toxicity determination,strain YN1-1 was screened as the target strain with wide spectrum,fast-acting property and high toxicity. |
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| Keywords: | Bacillus thuringiensis Biological determination Activity determination Toxicity determination |
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